Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (ab150132)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameDonkey Anti-Goat IgG H&L (Alexa Fluor® 594)
See all IgG secondary antibodies -
DescriptionDonkey polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 594)
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Host speciesDonkey
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Target speciesGoat
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SpecificityThis antibody is specific to Goat IgG
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Tested applicationsSuitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
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Immunogen
The details of the immunogen for this antibody are not available.
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ConjugationAlexa Fluor® 594. Ex: 590nm, Em: 617nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
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ClonalityPolyclonal
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IsotypeIgG
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab150132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | 1/2000. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (ab150132)ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150132 Alexa Fluor® 594 donkey anti-goat IgG (H+L) (orange) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody
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Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (ab150132)
References
This product has been referenced in:
- Zúñiga R et al. TASK-3 Downregulation Triggers Cellular Senescence and Growth Inhibition in Breast Cancer Cell Lines. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29596383) »
- Abe SI et al. Roles of CD34+ cells and ALK5 signaling in the reconstruction of seminiferous tubule-like structures in 3-D re-aggregate culture of dissociated cells from neonatal mouse testes. PLoS One 12:e0188705 (2017). IF ; Mouse . Read more (PubMed: 29190781) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"