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    donkey-goat-igg-hl-alexa-fluor-790-ab175784.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 790) (ab175784)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 790) (ab175784)

    Key features and details

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 790)
    • Conjugation: Alexa Fluor® 790. Ex: 782nm, Em: 805nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: WB

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

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    Overview

    • Product name

      Donkey Anti-Goat IgG H&L (Alexa Fluor® 790)
      See all IgG secondary antibodies
    • Host species

      Donkey
    • Target species

      Goat
    • Tested applications

      Suitable for: WBmore details
    • Immunogen

      Full length protein corresponding to Goat IgG.

    • Conjugation

      Alexa Fluor® 790. Ex: 782nm, Em: 805nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • General notes

      We batch test Donkey Anti-Goat IgG H&L (Alexa Fluor® 790), ab175784 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry, customers have had success using Donkey Anti-Goat IgG H&L (Alexa Fluor® 790), ab175784 in these applications.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Goat
      • IgG
      • Fluorophore
      • Alexa Fluor® 790

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab175784 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/10000.
    Notes
    WB
    1/10000.

    Images

    • Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 790) (ab175784)
      Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 790) (ab175784)
      Anti-GAPDH antibody - Loading Control (ab9483) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Donkey Anti-Goat IgG H&L (Alexa Fluor® 790) (ab175784) at 1/10000 dilution

      Observed band size: 37 kDa why is the actual band size different from the predicted?



      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab9483 overnight at 4°C. Antibody binding was detected using ab175784 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab175784? Please let us know so that we can cite the reference in this datasheet.

    ab175784 has been referenced in 2 publications.

    • Cosker K  et al. Microglial? signalling pathway deficits associated with the patient derived R47H TREM2 variants linked to AD indicate inability to activate inflammasome. Sci Rep 11:13316 (2021). PubMed: 34172778
    • Maxwell GK  et al. Developmental and degenerative cardiac defects in the Taiwanese mouse model of severe spinal muscular atrophy. J Anat 232:965-978 (2018). PubMed: 29473159

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Detection of PAX8 using LI-COR

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Good performance and quality. Specific and with a strong signal. Allows a big dilution and is still sensitive enough. I would recommend this antibody to anyone.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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