Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.10
    Preservative: 0.09% Sodium azide
    Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notes

    Molar enzyme: antibody protein ratio is 1:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/50000. (Primary).
WB 1/5000 - 1/50000. (Chemiluminescent).

Images

  • All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/5000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/20000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 8 minutes

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us with your question. For the alkaline phosphatase conjugated antibody ab97107, you can use the liquid fast-red kit ab64254- https://www.abcam.com/Liquid-Fast-Red-Substrate-Kit-ab64254.html For the HRP conjugated antibody ab97100, you can use the DAB kit ab64238- https://www.abcam.com/DAB-Substrate-Kit-ab64238.html I hope this information is helpful but please let me know if you have any questions.

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