Product nameDonkey Anti-Goat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.10
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Conjugation notesMolar enzyme: antibody protein ratio is 1:1
General notesPart of the AbExcel range.
Our Abpromise guarantee covers the use of ab97107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/5000 - 1/50000. (Primary).|
|WB||1/5000 - 1/50000. (Chemiluminescent).|
All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.
Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/5000 dilution
Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/20000 dilution
Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 8 minutes