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Could you suggest anti ab9871 secondary antibodies that can be sued in ELISA and ICC/IF?
Asked on Feb 02 2012
Thank you for contacting us.
As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.
Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However these will not work in Immunofluorescence unless further steps in protocol applied.
We have FITC, TRITC or Cy5 conjugated antibodies in catalogue that can work in Immunofluorescence and Fluorescence ELISA.
Another option is choosing Biotin conjugated secondary antibody and then buying strepavidin or streptavidin conjugated HRP (ab7403).
ab5753, ab6740, ab6884, ab7124, ab7131 coul dbe a good option.
I know it sounds complicated however this is only way to combine ELISA and ICC/IF with one secondary antibody.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Feb 02 2012