Key features and details
- Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameDonkey Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Chicken, Human, Mouse, Pig, Rabbit, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Other Immunogen Type corresponding to Goat IgG.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.10
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Our Abpromise guarantee covers the use of ab97112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/2500 - 1/25000. (as Primary).|
|WB||1/2500 - 1/25000. (Chemiluminescent).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97112 has been referenced in 2 publications.
- Hasegawa C et al. Establishment of an organ culture system to induce Sertoli cell differentiation from undifferentiated mouse gonads. J Vet Med Sci 82:414-421 (2020). PubMed: 32092744
- Yamamoto A et al. The mechanisms underlying the effects of AMH on Müllerian duct regression in male mice. J Vet Med Sci 80:557-567 (2018). PubMed: 29526868