Product nameDonkey Anti-Goat IgG H&L (Biotin)
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
Full length native Goat IgG (purified).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Use donkey anti-goat IgG (H&L) biotin to confidently detect primary antibodies raised in goat in your immunohistochemistry, western blot, ELISA or immunoprecipitation. Get dilutions to get the best results in your IHC, WB, IP, or ELISA with donkey anti-goat IgG (H&L) biotin below.
Our Abpromise guarantee covers the use of ab6884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|Dot blot||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
ab6884 used to stain samples of whole heads of embryonic day 15 (E15) mice by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
Comparison of four staining methods. The heads were flash-frozen directly in isopentane on dry-ice. Once hardened, they were embedded in O.C.T compound. Frozen sections were postfixed in 4% paraformaldehyde in PBS for 20 minutes and quenched in 1.5% hydrogen peroxide for 30 minutes. Sections were then blocked for 2 hours at room temperature with 5% donkey serum in Tris-buffered saline with 0.1% Triton-X100 and then incubated with a goat anti-Nurr1 primary antibody 1/200 in 1% donkey serum in TBS at 4°C overnight. Biotinylated donkey anti-goat (ab6884) 1/500 in 1% donkey serum in TBS was applied for 2 hours at room temperature and reacted with avidin-biotinylated enzyme complex (ABC) using the Vectastain Elite kit and diaminobenzidene (DAB) according to the manufacturer's instructions. Images were taken directly on the PALM Microbeam to demonstrate the staining and image quality available to identify the different cell populations.
Image a: Hematoxylin.Image b: Hematoxylin and Eosin.
Image c: 0.1% cresyl violet in H2O.Image d: 1% cresyl violet in H2O.
For hematoxylin and H&E staining (a, b), sections were rinsed with 70% EtOH and H2O, stained with Mayer's hematoxylin for 15 seconds, rinsed with H2O and 70% EtOH and stained with Accustain Eosin Y for 10 seconds (for H&E only). For cresyl violet staining (c, d), sections were rehydrated in decreasing concentrations of ethanol, stained in cresyl violet for 45 seconds and dehydrated in increasing concentrations of ethanol. 1% cresyl violet (d) provided the best morphological details and allowed clear distinction between cortical plate (CP) and subplate (SP).
This product has been referenced in:
- Johnson MB et al. Topical Fibronectin Improves Wound Healing of Irradiated Skin. Sci Rep 7:3876 (2017). IHC-P ; Mouse . Read more (PubMed: 28634413) »
- Neveu B et al. A PCA3 gene-based transcriptional amplification system targeting primary prostate cancer. Oncotarget 7:1300-10 (2016). Read more (PubMed: 26594800) »