Key features and details
- Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Product nameDonkey Anti-Goat IgG H&L (Biotin) preadsorbed
See all IgG secondary antibodies
DescriptionDonkey polyclonal Secondary Antibody to Goat IgG - H&L (Biotin), pre-adsorbed
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
Chicken, Human, Mouse, Pig, Rabbit, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Other Immunogen Type corresponding to Goat IgG.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Conjugation notesBiotin/ antibody protein ratio is 4:1
Our Abpromise guarantee covers the use of ab97113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/2500.|
|ICC/IF||1/100 - 1/500.|
|ELISA||1/10000 - 1/200000. (as Primary).|
|WB||1/10000 - 1/200000. (Chemiluminescent).|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97113 has been referenced in 1 publication.
- Boon J et al. Long-range projections from sparse populations of GABAergic neurons in murine subplate. J Comp Neurol 527:1610-1620 (2019). PubMed: 30520039