Donkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed (ab6948)

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Key features and details

  • Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy2 ®), pre-adsorbed
  • Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed
    See all IgG secondary antibodies

  • Description

    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy2 ®), pre-adsorbed

  • Host species

    Donkey

  • Target species

    Goat

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details

  • Minimal
    cross-reactivity


    Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation

    Cy2 ®. Ex: 489nm, Em: 505nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

  • Conjugation notes

    Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 3.9 moles Cy2 per mole of Donkey IgG

  • Clonality

    Polyclonal

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6948 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ELISA
    1/10000 - 1/50000.
    Notes
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ELISA
    1/10000 - 1/50000.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab6948? Please let us know so that we can cite the reference in this datasheet.

    ab6948 has been referenced in 1 publication.

    • Barsi JC  et al. Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech Dev 122:1106-17 (2005). PubMed: 16061358

    Customer reviews and Q&As

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    Question

    Dear TechTeam,

    Our customer has been waiting for ab97114 for 4 months and soonest delivery is indicated as in May 2012. Would it be possible to find alternative products, or maybe the one with label of close kind to Cy2?

    Many thanks,

    Read More
    Answer

    Thank you for your enquiry and you interest in our products. I am very sorry for the inconvenience caused.

    I am pleased to inform you that we have an alternative product and it is ab6948: Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy2 ®), pre-adsorbed. It is in stock and available.

    In future, I would advise you and your customers to use our advanced search engine designed for selecting compatible secondary antibodies: https://www.abcam.com/index.html?pageconfig=productmap&cl=918

    You can type the isotype, target species, host species, format, specificity, conjugates and search for the most suitable products.

    If you need any further assistance in the future, please do not hesitate to contact me.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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