Donkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed (ab6948)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameDonkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed
See all IgG secondary antibodies -
DescriptionDonkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy2 ®), pre-adsorbed
-
Host speciesDonkey
-
Target speciesGoat
-
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
-
Minimal
cross-reactivity
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
Immunogen
Full length native Goat IgG (purified).
-
ConjugationCy2 ®. Ex: 489nm, Em: 505nm
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
-
Conjugation notesCy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 3.9 moles Cy2 per mole of Donkey IgG
-
ClonalityPolyclonal
-
General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
-
Research areas
Applications
Our Abpromise guarantee covers the use of ab6948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent dilution. | |
| ELISA | 1/10000 - 1/50000. |
References
This product has been referenced in:
- Barsi JC et al. Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech Dev 122:1106-17 (2005). Read more (PubMed: 16061358) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"