Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WB
Related conjugates and formulations
Alexa Fluor® 405
Alexa Fluor® 488
Alexa Fluor® 555
Alexa Fluor® 568
Alexa Fluor® 594
Alexa Fluor® 647
Alexa Fluor® 750
Alexa Fluor® 790
Alkaline Phosphatase
Biotin
Cy2 ®
Cy5 ®
Cy5.5 ®
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 650
FITC
Gold 10nm
Gold 12nm
Gold 18nm
Gold 25nm
HRP
HRP polymer
IRDye® 800CW
PE
Texas Red ®
TRITC
Unconjugated
Unconjugated
Unconjugated
Overview
-
Product name
Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WBmore details -
Minimal
cross-reactivity
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
Immunogen
Full length native Goat IgG (purified).
-
Conjugation
Cy3 ®. Ex: 552nm, Em: 565nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
Conjugation notes
Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
-
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| ICC |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
1/1000. Predicted molecular weight: 36 kDa.
|
| Notes |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
|
WB
1/1000. Predicted molecular weight: 36 kDa. |
Images
-
Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949) at 1000 cells + Goat IgG, 50 ng
Predicted band size: 36 kDaBoth the heavy and light chains of Goat IgG are observed.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (28)
ab6949 has been referenced in 28 publications.
- Tian C & Johnson KR TBX1 is required for normal stria vascularis and semicircular canal development. Dev Biol 457:91-103 (2020). PubMed: 31550482
- Gao L et al. Vps35 Deficiency Impairs Cdk5/p35 Degradation and Promotes the Hyperphosphorylation of Tau Protein in Retinal Ganglion Cells. Invest Ophthalmol Vis Sci 61:1 (2020). PubMed: 31995153
- Mahameed M et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun 11:1304 (2020). PubMed: 32161259
- Cooke JE et al. Contrast gain control occurs independently of both parvalbumin-positive interneuron activity and shunting inhibition in auditory cortex. J Neurophysiol 123:1536-1551 (2020). PubMed: 32186432
- Srivastava Y et al. Cancer-associated missense mutations enhance the pluripotency reprogramming activity of OCT4 and SOX17. FEBS J 287:122-144 (2020). PubMed: 31569299