Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WB
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Overview
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Product name
Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WBmore details -
Minimal
cross-reactivity
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
Immunogen
Full length native Goat IgG (purified).
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Conjugation
Cy3 ®. Ex: 552nm, Em: 565nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
Conjugation notes
Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| ICC |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
1/1000. Predicted molecular weight: 36 kDa.
|
| Notes |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
|
WB
1/1000. Predicted molecular weight: 36 kDa. |
Images
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Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949) at 1000 cells + Goat IgG, 50 ng
Predicted band size: 36 kDaBoth the heavy and light chains of Goat IgG are observed.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab6949 has been referenced in 32 publications.
- Xu Y et al. Caffeine Functions by Inhibiting Dorsal and Ventral Hippocampal Adenosine 2A Receptors to Modulate Memory and Anxiety, Respectively. Front Pharmacol 13:807330 (2022). PubMed: 35185566
- Mohammad G et al. The kidney hepcidin/ferroportin axis controls iron reabsorption and determines the magnitude of kidney and systemic iron overload. Kidney Int 100:559-569 (2021). PubMed: 33991530
- Wang JY et al. Mechanisms underlying antidepressant effect of transcutaneous auricular vagus nerve stimulation on CUMS model rats based on hippocampal a7nAchR/NF-?B signal pathway. J Neuroinflammation 18:291 (2021). PubMed: 34920740
- Srivastava Y et al. Cancer-associated missense mutations enhance the pluripotency reprogramming activity of OCT4 and SOX17. FEBS J 287:122-144 (2020). PubMed: 31569299
- Zhao Q et al. Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output. Cell 183:76-93.e22 (2020). PubMed: 32931733