Key features and details
- Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3 ®), pre-adsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WB
Product nameDonkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
See all IgG secondary antibodies
DescriptionDonkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3 ®), pre-adsorbed
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WBmore details
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native Goat IgG (purified).
ConjugationCy3 ®. Ex: 552nm, Em: 565nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Conjugation notesCy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG
General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Our Abpromise guarantee covers the use of ab6949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
|WB||1/1000. Predicted molecular weight: 36 kDa.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6949 has been referenced in 21 publications.
- Kuehn S et al. Interaction of complement system and microglia activation in retina and optic nerve in a NMDA damage model. Mol Cell Neurosci 89:95-106 (2018). PubMed: 29738834
- Chu P et al. The Impact of Perineuronal Net Digestion Using Chondroitinase ABC on the Intrinsic Physiology of Cortical Neurons. Neuroscience 388:23-35 (2018). PubMed: 30004010
- Veerapandian V et al. Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing. Stem Cell Reports 11:593-606 (2018). PubMed: 30078555
- Wang X et al. Inhibition of overactive TGF-ß attenuates progression of heterotopic ossification in mice. Nat Commun 9:551 (2018). PubMed: 29416028
- Pfanzagl B et al. Activation of the ileal neuroendocrine tumor cell line P-STS by acetylcholine is amplified by histamine: role of H3R and H4R. Sci Rep 7:1313 (2017). PubMed: 28465562