• Product name
    Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3 ®), pre-adsorbed
  • Host species
  • Target species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WBmore details
  • Minimal

    Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation
    Cy3 ®. Ex: 552nm, Em: 565nm


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
  • Conjugation notes
    Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG
  • Clonality
  • Isotype
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.

    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas


Our Abpromise guarantee covers the use of ab6949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
WB 1/1000. Predicted molecular weight: 36 kDa.


  • Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949) at 1000 cells + Goat IgG, 50 ng

    Predicted band size: 36 kDa

    Both the heavy and light chains of Goat IgG are observed.


This product has been referenced in:
  • Kuehn S  et al. Interaction of complement system and microglia activation in retina and optic nerve in a NMDA damage model. Mol Cell Neurosci 89:95-106 (2018). Read more (PubMed: 29738834) »
  • Chu P  et al. The Impact of Perineuronal Net Digestion Using Chondroitinase ABC on the Intrinsic Physiology of Cortical Neurons. Neuroscience 388:23-35 (2018). Read more (PubMed: 30004010) »
See all 18 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (PFA fixed)
I used this secondary antibody on PFA-fixed frozen brain sections to bind to the primary antibody sox 2 (goat).

Abcam user community

Verified customer

Submitted Dec 08 2014


I'm not sure why you would want to use this secondary antibody conjugated to a fluorophore in Western Blotting. The secondary would bind to your goat primary, but you would not easily be able to detect it. I would definitely not recommend your using this antibody in WB. I would suggest a secondary conjugated to hrp followed by ECL.

Read More


Sign up