Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)


  • Product name
    Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed
  • Host species
  • Target species
  • Tested applications
    Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details
  • Minimal

    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Goat IgG, whole molecule

  • Conjugation
    Cy5 ®. Ex: 650nm, Em: 667nm


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
  • Conjugation notes
    Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
  • Clonality
  • Isotype
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.

    This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas


Our Abpromise guarantee covers the use of ab6566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.


  • Lanes 2-3 : Goat anti-UCP1 at 1/200 dilution

    Lane 1 : Marker
    Lanes 2-3 : Mouse brown adipose tissue lysate at 70 µg

    Lanes 2-3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution

    Observed band size: 33 kDa (why is the actual band size different from the predicted?)


This product has been referenced in:
  • Menges S  et al. Alpha-synuclein prevents the formation of spherical mitochondria and apoptosis under oxidative stress. Sci Rep 7:42942 (2017). Read more (PubMed: 28224980) »
  • Kuo WY  et al. STAT3/NF-?B-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer. Theranostics 7:647-663 (2017). Read more (PubMed: 28255357) »

See all 15 Publications for this product

Customer reviews and Q&As

Western blot
All lanes: Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) 1:2500
Lane 1: Marker
Lane 2: Protein lysate of BAT (Mouse 1), Protein 70µg
Lane 3: Protein lysate of BAT (Mouse 2), Protein 70µg
Lanes 2+3: Primary Antibody anti-UCP1 1:200
Predicted band size 33kDa

Abcam user community

Verified customer

Submitted Aug 20 2014

Thank you for your enquiry. I believe Cy5.29 OSu is the same as Cy5, but refers more specifically to the cyanine derivative, a succinimide ester, that is used for conjugation. The following paper describes this ester: Cytometry. 1990;11(3):418-3...

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This antibody will indeed recognise the other secondary. You need to use a secondary antibody to the mouse IgG raised in a species other than goat, preferably donkey (if you want to use ab6566). Look for a donkey anti-mouse IgG fluorphore conjugated se...

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