Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Goat
  • Tested applications

    Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Goat IgG, whole molecule

  • Conjugation

    Cy5 ®. Ex: 650nm, Em: 667nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
  • Conjugation notes

    Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

Images

  • Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 µm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 µm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.

    Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).

  • Lanes 2-3 : Goat anti-UCP1 at 1/200 dilution

    Lane 1 : Marker
    Lanes 2-3 : Mouse brown adipose tissue lysate at 70 µg

    Secondary
    Lanes 2-3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution

    Observed band size: 33 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Lei JJ  et al. Long noncoding RNA CDKN2B-AS1 interacts with transcription factor BCL11A to regulate progression of cerebral infarction through mediating MAP4K1 transcription. FASEB J 33:7037-7048 (2019). Read more (PubMed: 30870006) »
  • Yoshida Y  et al. Peptide vaccine for semaphorin3E ameliorates systemic glucose intolerance in mice with dietary obesity. Sci Rep 9:3858 (2019). Read more (PubMed: 30846754) »
See all 19 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
All lanes: Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) 1:2500
Lane 1: Marker
Lane 2: Protein lysate of BAT (Mouse 1), Protein 70µg
Lane 3: Protein lysate of BAT (Mouse 2), Protein 70µg
Lanes 2+3: Primary Antibody anti-UCP1 1:200
Predicted band size 33kDa

Abcam user community

Verified customer

Submitted Aug 20 2014

Answer

Thank you for your enquiry. I believe Cy5.29 OSu is the same as Cy5, but refers more specifically to the cyanine derivative, a succinimide ester, that is used for conjugation. The following paper describes this ester: Cytometry. 1990;11(3):418-30. PMID: 2340776 I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

This antibody will indeed recognise the other secondary. You need to use a secondary antibody to the mouse IgG raised in a species other than goat, preferably donkey (if you want to use ab6566). Look for a donkey anti-mouse IgG fluorphore conjugated secondary antibody on the Abcam website.

Read More

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