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  1. Link

    donkey-goat-igg-hl-cy55--preadsorbed-ab6951.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed (ab6951)

  • Datasheet
Submit a review Q&A (1)References (7)

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Key features and details

  • Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed
  • Conjugation: Cy5.5 ®. Ex: 678nm, Em: 703nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity


    Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation

    Cy5.5 ®. Ex: 678nm, Em: 703nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
  • Conjugation notes

    Cy5.5 (Cyanine 5.5-OSu) (Molecular Weight 1312 daltons) Absorption Wavelength: 678 nm Emission Wavelength: 703 nm Fluorochrome/Protein Ratio: 4.1 moles Cy5.5 per mole of Donkey IgG
  • Clonality

    Polyclonal
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Fluorophore
    • Cy5.5

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
Notes
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (7)

Publishing research using ab6951? Please let us know so that we can cite the reference in this datasheet.

ab6951 has been referenced in 7 publications.

  • Bauwens S  et al. The Telomeric Protein TRF2 Regulates Replication Origin Activity within Pericentromeric Heterochromatin. Life (Basel) 11:N/A (2021). PubMed: 33804994
  • Hernandes MS  et al. Polymerase delta-interacting protein 2 deficiency protects against blood-brain barrier permeability in the ischemic brain. J Neuroinflammation 15:45 (2018). PubMed: 29452577
  • Mendez-Bermudez A  et al. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2. Mol Cell 70:449-461.e5 (2018). PubMed: 29727617
  • Wilhelm T  et al. Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells. Proc Natl Acad Sci U S A 111:763-8 (2014). PubMed: 24347643
  • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). ICC/IF . PubMed: 24726359
  • Técher H  et al. Replication dynamics: biases and robustness of DNA fiber analysis. J Mol Biol 425:4845-55 (2013). PubMed: 23557832
  • Letessier A  et al. Cell-type-specific replication initiation programs set fragility of the FRA3B fragile site. Nature 470:120-3 (2011). IF . PubMed: 21258320

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Any idea how long the Cy5.5 can be visualized after IHC staining procedure? Or does it just depend on length of time exposed to laser? What is best way to store sections stained with Cy5.5 conjugated secondary if I want to image in the future?

Read More

Abcam community

Verified customer

Asked on Jan 14 2013

Answer

Unfortunately we do not have data on the stability of C5.5 dyes on fixed stored IHC slides. This would depend on how they were imaged and the mounting medium that was used, as well as the exposure of the slides to light, however.
.

Read More

Abcam Scientific Support

Answered on Jan 14 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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