Overview

  • Product name
    Donkey Anti-Goat IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Goat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Tang S  et al. Apolipoprotein A-I enhances insulin-dependent and insulin-independent glucose uptake by skeletal muscle. Sci Rep 9:1350 (2019). Read more (PubMed: 30718702) »
  • Tschurtschenthaler M  et al. Defective ATG16L1-mediated removal of IRE1a drives Crohn's disease-like ileitis. J Exp Med 214:401-422 (2017). Read more (PubMed: 28082357) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us and your interest in our products.

I am sorry for the delay in getting back to you. We have quite a few antibodies which should be suitable for your experiment and should reduce the level of non-specific staining you are currently observing. The following antibodies have all been pre-adsorbed to reduce the cross reactivity with rabbit IgG (as well as IgG from other species):

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy3.5 ®), pre-adsorbed (ab6950)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5.5 ®), pre-adsorbed (ab6951)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy2 ®), pre-adsorbed (ab6948)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed (ab96935)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 594), pre-adsorbed (ab96937)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 650), pre-adsorbed (ab96938)

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed (ab97120)

If there is any other conjugate you would prefer for your experiment please let me know and I can have a look to see if we have one which has been pre-adsorbed to reduce cross reactivity with rabbit.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

Read More

Answer

Thank you for your reply. I'm glad my email reached you.

Yes, the donkey anti-Goat IgG H&L (Biotin) secondary antibody (ab7124) should also work fine for the experiment you are planning.

If I can be of any further help, please do let me know. Until then, I wish you all the best with your research.

Read More

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