Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)

Reviews (1)Q&A (5)References (6)
Western blot - Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
  • Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)

Key features and details

  • Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed
  • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: Flow Cyt, IHC-P, ICC/IF, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies

  • Description

    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed

  • Host species

    Donkey

  • Target species

    Goat

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details

  • Minimal
    cross-reactivity


    Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details

  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50 - 1/200.
IHC-P
1/50 - 1/500.
ICC/IF
1/50 - 1/500.
WB
1/1000 - 1/2000. Predicted molecular weight: 36 kDa.

5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.
Notes
Flow Cyt
1/50 - 1/200.
IHC-P
1/50 - 1/500.
ICC/IF
1/50 - 1/500.
WB
1/1000 - 1/2000. Predicted molecular weight: 36 kDa.

5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.

Images

  • Western blot - Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
    Western blot - Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
    All lanes : cocktail of mouse anti-hnRNP
    , rabbit anti-actin, and goat anti-GAPDH at 1 µg/ml

    All lanes : Hela

    Secondary
    All lanes : cocktail of Dylight® 680-conjugated donkey anti-mouse
    (green), Dylight® 800-
    conjugated donkey anti-rabbit (red), and Dylight® 488-conjugated
    donkey anti-goat (blue) (ab96935) at 0.5 µg/ml

    Predicted band size: 36 kDa

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)Image is courtesy of an AbReview submitted by Ms Francoise Geffroy.

    Immunohistochemical analysis of Paraformaldehyde perfusion-fixed frozen human neuroblastoma xenograft into a maurine host. The image is labelling LIMPII with ab87637 at 20°C for 1 hour at a dilution of 1/50 with 5% Donkey Serum. Permeabilization was done with 0.2% Triton. Secondary used was ab96935 at a 1/250 dilution.

    See Abreview

  • Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
    Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (6)

Publishing research using ab96935? Please let us know so that we can cite the reference in this datasheet.

ab96935 has been referenced in 6 publications.

  • Gentile SD  et al. Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids. Front Bioeng Biotechnol 8:792 (2020). PubMed: 32793571
  • Kaylan KB  et al. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling. Elife 7:N/A (2018). PubMed: 30589410
  • Compeer EB  et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun 9:1597 (2018). PubMed: 29686427
  • Rodriguez AM & Downs KM Visceral endoderm and the primitive streak interact to build the fetal-placental interface of the mouse gastrula. Dev Biol N/A:N/A (2017). PubMed: 28882402
  • Kawamura M  et al. Tissue-engineered smooth muscle cell and endothelial progenitor cell bi-level cell sheets prevent progression of cardiac dysfunction, microvascular dysfunction, and interstitial fibrosis in a rodent model of type 1 diabetes-induced cardiomyopathy. Cardiovasc Diabetol 16:142 (2017). PubMed: 29096622
  • Kaylan KB  et al. Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFß, and extracellular matrix. Sci Rep 6:23490 (2016). PubMed: 27025873

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat brains were fixed with paraformaldehyde 4%. Frozen section were realised (30µm).
Tissue sections were blocked with serum goat 5% + BSA 1% + Triton 0.2% during 2h.
First antibody was applied 1h (Iba-1 ref ab 5076) diluted 1/500, sections were washed twice with PBS.
Second antibody (ab 96935) was applied 1h , diluted 1/250, sections were washed again 3 time, before mounting with appropried medium.

Mrs. Francoise Geffroy

Verified customer

Submitted Aug 22 2014

Question

Will it be possible to give Abpromise for IHC-Fr with ab96935 in this particular case, since this will be helpfulin "closing the deal" with the customer?

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Answer

Thank you for your message.
Yes, your customer can get a discount code for writing an Abreview about this secondary antibody. However, as always, we would need their email address and institute for our records.
I am looking forward to hearing from you with the requested details.

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Question

Inquiry: Kindly advise if ab96935 can be used also in IHC-Fr. It is not mentioned in the tested application but do you see any reason that this will not perform in IHC-Fr? Thank you for the assistance and reply.

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Answer

Thank you for contacting us.
You are right, this shouldn't be a problem in this case, as the "target protein" is not been altered due to fixation etc. In addition, the antibody does work in tissue already, therefore, it should work.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

thank you for your answer. The order we made was at your supplier in Italy from November 2011. I attach here the order we submitted to them. The order included also a primary antibody for mu-opioid receptor, but my only problem is with the secondary antibody. I have to say that the approximation of antibody quantity is virtually zero cause it's impossible to take any aliquot from the tube using a Gilson. If you can do something about it, please, the shipping address is the same of the invoice:

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Answer

Thank you for contacting Abcam. I am sorry that the product you received was not of the full volume. I have processed a free of charge replacement for you. You should be receiving information from your supplier shortly. Please feel free to contact me should you have any questions.

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Question

Dear Tech Support Team, Our customer would like to use ab96935 for IHC-Fr with mouse sections. Unfortunately, none of anti goat, DyLight488, pre-adsorbed was tested in IHC-Fr. From your experience, do you think that ab96935 will work with frozen sections? Will it be possible to proceed with abreview promotion for ab96935 or you will give Abpromise for ab96935 for IHC-Fr as an exception in this case?Thank you for your assistance and cooperation.

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Answer

Thank you very much for contacting us. Indeed, we assume that ab96935 should work with frozen sections. Having spoken with our Abreview department, I am happy to let you know that the customer is eligible for our testing discount promotion: Similar to our Abreview promotion, we can offer a discount off a future purchase if the customer buys ab96935 now, test it in IHC-Fr and submit feedback to us. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive the feedback (images are great if they have them!). The discount would be to the value of 1 free secondary antibody. To let us know the results, positive or negative, please send an email to us (technical@abcam.com) and mention this exceptional promotion. As usual, after the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any secondary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab96935 turns out to be unsuitable for IHC-Fr, the customer will still receive the discount on the next purchase after the feedback has been submitted. Please let me know if you have any questions about this offer or if you would like to receive the discount code, and I would be happy to help you further.

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Question

Dear Abcam, I'm having a problem with your secondary antibody, the datasheet says it's 500 micrograms in liquid form at a concentrationof 0,5 mg/ml, but in truth it seems it has to be reconstituted cause there isn't 1ml of antibody in the tube, but datasheet doesn't say how I should reconstitute it. How could I do it? Can you help me? Thank you very much,

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Answer

Thank you for contacting us. I am sorry for the trouble caused here. This product is shipped in a ready-to-use form so reconstitution is unneccessary. Since you have not received the entire amount, 1ml, I would like to send you a free of charge replacement. If you could please send me the order number or po number used to purchase this product I can begin processing that for you. Would you also be able to send me an approximation of how much antibody was actually delivered for our records? I look forward to hearing from you. Please do not hesitate to contact us if you need any more advice or information.

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