Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)

Abreviews (1)Q&A (5)Specific References (3)

Overview

  • Product name
    Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed
  • Host species
    Donkey
  • Target species
    Goat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity

    Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.

Images

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)Image is courtesy of an AbReview submitted by Ms Francoise Geffroy.

    Immunohistochemical analysis of Paraformaldehyde perfusion-fixed frozen human neuroblastoma xenograft into a maurine host. The image is labelling LIMPII with ab87637 at 20°C for 1 hour at a dilution of 1/50 with 5% Donkey Serum. Permeabilization was done with 0.2% Triton. Secondary used was ab96935 at a 1/250 dilution.

    See Abreview

  • DyLight®-Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed(ab96935)
    DyLight®-Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed(ab96935)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Compeer EB  et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun 9:1597 (2018). Read more (PubMed: 29686427) »
  • Kawamura M  et al. Tissue-engineered smooth muscle cell and endothelial progenitor cell bi-level cell sheets prevent progression of cardiac dysfunction, microvascular dysfunction, and interstitial fibrosis in a rodent model of type 1 diabetes-induced cardiomyopathy. Cardiovasc Diabetol 16:142 (2017). Read more (PubMed: 29096622) »

See all 3 Publications for this product

Publishing research using ab96935? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

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Votes: Highest First

Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed

 Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat brains were fixed with paraformaldehyde 4%. Frozen section were realised (30µm).
Tissue sections were blocked with serum goat 5% + BSA 1% + Triton 0.2% during 2h.
First antibody was applied 1h (Iba-1 ref ab 5076) diluted 1/500, sections were washed twice with PBS.
Second antibody (ab 96935) was applied 1h , diluted 1/250, sections were washed again 3 time, before mounting with appropried medium.
abreview image
Username

Mrs. Francoise Geffroy

Verified customer

Submitted Aug 22 2014

Will it be possible to give Abpromise for IHC-Fr with ab96935 in this particular case, since this will be helpfulin "closing the deal" with the customer?

Thank you for your message.
Yes, your customer can get a discount code for writing an Abreview about this secondary antibody. However, as always, we would need their email address and institute for our records.
I am looking forward to hearing...

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Inquiry: Kindly advise if ab96935 can be used also in IHC-Fr. It is not mentioned in the tested application but do you see any reason that this will not perform in IHC-Fr? Thank you for the assistance and reply.

Thank you for contacting us.
You are right, this shouldn't be a problem in this case, as the "target protein" is not been altered due to fixation etc. In addition, the antibody does work in tissue already, therefore, it should work.
I hope th...

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thank you for your answer. The order we made was at your supplier in Italy from November 2011. I attach here the order we submitted to them. The order included also a primary antibody for mu-opioid receptor, but my only problem is with the secondary a...

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Thank you for contacting Abcam. I am sorry that the product you received was not of the full volume. I have processed a free of charge replacement for you. You should be receiving information from your supplier shortly. Please feel free to contact me...

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Dear Tech Support Team, Our customer would like to use ab96935 for IHC-Fr with mouse sections. Unfortunately, none of anti goat, DyLight488, pre-adsorbed was tested in IHC-Fr. From your experience, do you think that ab96935 will work with frozen sectio...

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Thank you very much for contacting us. Indeed, we assume that ab96935 should work with frozen sections. Having spoken with our Abreview department, I am happy to let you know that the customer is eligible for our testing discount promotion: Similar to ...

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Dear Abcam, I'm having a problem with your secondary antibody, the datasheet says it's 500 micrograms in liquid form at a concentrationof 0,5 mg/ml, but in truth it seems it has to be reconstituted cause there isn't 1ml of antibody in the tube, but dat...

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Thank you for contacting us. I am sorry for the trouble caused here. This product is shipped in a ready-to-use form so reconstitution is unneccessary. Since you have not received the entire amount, 1ml, I would like to send you a free of charge replac...

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