Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed (ab96935)

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 488), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity


    Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.

Images

  • Immunohistochemical analysis of Paraformaldehyde perfusion-fixed frozen human neuroblastoma xenograft into a maurine host. The image is labelling LIMPII with ab87637 at 20°C for 1 hour at a dilution of 1/50 with 5% Donkey Serum. Permeabilization was done with 0.2% Triton. Secondary used was ab96935 at a 1/250 dilution.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Compeer EB  et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. Nat Commun 9:1597 (2018). Read more (PubMed: 29686427) »
  • Kaylan KB  et al. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling. Elife 7:N/A (2018). Read more (PubMed: 30589410) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat brains were fixed with paraformaldehyde 4%. Frozen section were realised (30µm).
Tissue sections were blocked with serum goat 5% + BSA 1% + Triton 0.2% during 2h.
First antibody was applied 1h (Iba-1 ref ab 5076) diluted 1/500, sections were washed twice with PBS.
Second antibody (ab 96935) was applied 1h , diluted 1/250, sections were washed again 3 time, before mounting with appropried medium.

Mrs. Francoise Geffroy

Verified customer

Submitted Aug 22 2014

Answer

Thank you for your message.
Yes, your customer can get a discount code for writing an Abreview about this secondary antibody. However, as always, we would need their email address and institute for our records.
I am looking forward to hearing from you with the requested details.

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Answer

Thank you for contacting us.
You are right, this shouldn't be a problem in this case, as the "target protein" is not been altered due to fixation etc. In addition, the antibody does work in tissue already, therefore, it should work.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting Abcam. I am sorry that the product you received was not of the full volume. I have processed a free of charge replacement for you. You should be receiving information from your supplier shortly. Please feel free to contact me should you have any questions.

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Answer

Thank you very much for contacting us. Indeed, we assume that ab96935 should work with frozen sections. Having spoken with our Abreview department, I am happy to let you know that the customer is eligible for our testing discount promotion: Similar to our Abreview promotion, we can offer a discount off a future purchase if the customer buys ab96935 now, test it in IHC-Fr and submit feedback to us. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive the feedback (images are great if they have them!). The discount would be to the value of 1 free secondary antibody. To let us know the results, positive or negative, please send an email to us (technical@abcam.com) and mention this exceptional promotion. As usual, after the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any secondary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab96935 turns out to be unsuitable for IHC-Fr, the customer will still receive the discount on the next purchase after the feedback has been submitted. Please let me know if you have any questions about this offer or if you would like to receive the discount code, and I would be happy to help you further.

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Answer

Thank you for contacting us. I am sorry for the trouble caused here. This product is shipped in a ready-to-use form so reconstitution is unneccessary. Since you have not received the entire amount, 1ml, I would like to send you a free of charge replacement. If you could please send me the order number or po number used to purchase this product I can begin processing that for you. Would you also be able to send me an approximation of how much antibody was actually delivered for our records? I look forward to hearing from you. Please do not hesitate to contact us if you need any more advice or information.

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