Overview

  • Product name
    Donkey Anti-Goat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Goat
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, WBmore details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.
WB 1/5000 - 1/20000.

Images

  • Ab6881 was used at dilution 1/200 with the primary antibody ab27985 in Flow Cyt. See the review on ab27985.
  • Ab6881 was used at dilution 1/500 with the primary antibody ab26234 in ICC/IF. See the review on ab26234.

References

This product has been referenced in:
  • Kuang Y  et al. Cluster of differentiation 147 mediates chemoresistance in breast cancer by affecting vacuolar H+-ATPase expression and activity. Oncol Lett 15:7279-7290 (2018). Read more (PubMed: 29731886) »
  • Giacomini A  et al. Treatment with corn oil improves neurogenesis and cognitive performance in the Ts65Dn mouse model of Down syndrome. Brain Res Bull 140:378-391 (2018). Read more (PubMed: 29935232) »
See all 21 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Merci de nous avoir contactés.

Vous avez du recevoir dans un autre email un devis pour l'anticorps ab6881.

J'ai reçu confirmation du laboratoire que l'anticorps ab6881 fonctionne en Western Blot. Les dilutions recommandées sont 1/5000 - 1/20000. La fiche technique a été mise à jour avec ces informations (https://www.abcam.com/ab6881).

Comme nous avons plus de 90.000 références, nous ne pouvons stocker différents volumes pour nos produits et nous n'offrons donc pas d'échantillon gratuit ou de petit volume pour des essais préliminaires.

Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique, nous offrons un remboursement ou un remplacement gratuit.

J'espère que ces informations vous aideront. N'hésitez pas à me contacter si vous avez besoin de plus de renseignements.

Read More

Answer

Thank you for your protocol and your order information, we have sold only 1 vial of anti-Foxp3 to a customer in Cape town but the address is very different and it was bought in March, could this be you? I have carefully looked at each step of your protocol and would recommend the following modifications: -you say "defrost after fixing" and then mention fixing again. Can I please make sure the sections are fresh frozen and not perfusion fixed. Fresh frozen sections should be then postfixed for 20min in 4% paraformaldehyde in PB, or for 10min in ice cold acetone. -I recommend washing slides in PBS 3-4 times between each step, to decrease non specific binding -use the dilution buffer PBST to dilute the blocking agent and antibodies. PBST is made of PBS + 0.3% v/v TritonX100, to permeabilise the cells. Even if your sections are very thin they may need permeabilisation (if you fix with acetone this is not necessary as acetone fixes and permeabilises too) -block in normal serum 10% rather than BSA, this can give problems in some rare cases - try to use biotin-ABC-DAB rather than IF, as this is more specific and more sensitive. I hope these suggestions will help you, please let me know how you get on and good luck with your experiments,

Read More

Answer

Thank you for your email. For PERCP, do you mean the tandem conjugate PerCP-Cy5.5? I'm not sure what you mean by Sycron so it would great if you could provide me with some more information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up