Overview

  • Product name
    Donkey Anti-Goat IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Goat
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, WBmore details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.
WB 1/5000 - 1/20000.

Images

  • Ab6881 was used at dilution 1/200 with the primary antibody ab27985 in Flow Cyt. See the review on ab27985.
  • Ab6881 was used at dilution 1/500 with the primary antibody ab26234 in ICC/IF. See the review on ab26234.

References

This product has been referenced in:
  • Kuang Y  et al. Cluster of differentiation 147 mediates chemoresistance in breast cancer by affecting vacuolar H+-ATPase expression and activity. Oncol Lett 15:7279-7290 (2018). Read more (PubMed: 29731886) »
  • Liu J  et al. Homemade-device-induced negative pressure promotes wound healing more efficiently than VSD-induced positive pressure by regulating inflammation, proliferation and remodeling. Int J Mol Med 39:879-888 (2017). WB . Read more (PubMed: 28290607) »

See all 18 Publications for this product

Customer reviews and Q&As

Merci de nous avoir contactés.

Vous avez du recevoir dans un autre email un devis pour l'anticorps ab6881.

J'ai reçu confirmation du laboratoire que l'anticorps ab6881 fonctionne en Western Blot. Les dilutions recommand&...

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Thank you for your protocol and your order information, we have sold only 1 vial of anti-Foxp3 to a customer in Cape town but the address is very different and it was bought in March, could this be you? I have carefully looked at each step of your ...

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Thank you for your email. For PERCP, do you mean the tandem conjugate PerCP-Cy5.5? I'm not sure what you mean by Sycron so it would great if you could provide me with some more information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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