Overview

  • Product name

    Donkey Anti-Goat IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Tested applications

    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity


    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
ELISA
IHC-P
IHC-Fr
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:

    • Baird A  et al. Osteoblast differentiation of equine induced pluripotent stem cells. Biol Open 7:N/A (2018). Read more (PubMed: 29685993) »
    • Raghuram GV  et al. Physical shearing imparts biological activity to DNA and ability to transmit itself horizontally across species and kingdom boundaries. BMC Mol Biol 18:21 (2017). Read more (PubMed: 28793862) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    FITC-labelled antibodies are stable for at least 6 months at room temperature, although there may be a slight decrease in activity after 3 months. Storage at +4oC, will increase the shelf-life.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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