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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Goat IgG H&L (HRP) (ab205723)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (9)

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Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab205723)

Key features and details

  • Donkey Anti-Goat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IP, WB, IHC-P, ELISA

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Overview

  • Product name

    Donkey Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Goat
  • Specificity

    The antibody used for conjugation reacts with goat immunoglobulins of all classes. Cross-reactions as determined by ELISA for the unconjugated antibody (ab182021): Human IgG, mouse IgG, rat IgG, rabbit IgG and chicken IgY, less than 2%.
  • Tested applications

    Suitable for: IP, WB, IHC-P, ELISAmore details
  • Immunogen

    Full length protein corresponding to Goat IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Enzyme
    • HRP

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab205723 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at an assay dependent concentration.
    WB 1/2000 - 1/20000.
    IHC-P 1/2000 - 1/20000.
    ELISA Use at an assay dependent concentration.

    Images

    • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab205723)
      Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab205723)
      All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml

      Lane 1 : Liver (Human) Tissue Lysate
      Lane 2 : Liver (Mouse) Tissue Lysate
      Lane 3 : Liver (Rat) Tissue Lysate
      Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 42 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using ab205723, and visualised using ECL development solution ab133406.

    • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab205723)
      Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab205723)
      All lanes : No Primary Antibody

      Lane 1 : Liver (Human) Tissue Lysate
      Lane 2 : Liver (Mouse) Tissue Lysate
      Lane 3 : Liver (Rat) Tissue Lysate
      Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1/2000 dilution

      Performed under reducing conditions.

      Exposure time: 5 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was incubated overnight with 2% Bovine Serum Albumin at 4°C. Any non-specific background binding was assessed by incubating the membrane with only the secondary antibody (ab205723), and visualised using ECL development solution ab133406.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab205723)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab205723)

      IHC image of alpha tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab7291 at 1ug/ml. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h.

      An HRP-conjugated secondary (Ab205723, 1/2000 dilution) was used to detect the primary for 1hr at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (9)

    Publishing research using ab205723? Please let us know so that we can cite the reference in this datasheet.

    ab205723 has been referenced in 9 publications.

    • Cai X  et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). PubMed: 31190436
    • Pan J  et al. lncRNA ZFAS1 Is Involved in the Proliferation, Invasion and Metastasis of Prostate Cancer Cells Through Competitively Binding to miR-135a-5p. Cancer Manag Res 12:1135-1149 (2020). PubMed: 32104094
    • Giraud E  et al. Osteopontin in the host response to Leishmania amazonensis. BMC Microbiol 19:32 (2019). PubMed: 30736736
    • Fu Q  et al. Analysis of clinical characteristics of macrophage capping protein (CAPG) gene expressed in glioma based on TCGA data and clinical experiments. Oncol Lett 18:1344-1350 (2019). PubMed: 31423196
    • Su T  et al. LIM homeodomain transcription factor Isl1 affects urethral epithelium differentiation and apoptosis via Shh. Cell Death Dis 10:713 (2019). PubMed: 31558700
    • Zhou R  et al. Recombinant CC16 inhibits NLRP3/caspase-1-induced pyroptosis through p38 MAPK and ERK signaling pathways in the brain of a neonatal rat model with sepsis. J Neuroinflammation 16:239 (2019). PubMed: 31775794
    • Mao X  et al. Up-regulated Linc00472 suppresses development of lung cancer cell via inhibition of MiR-196b-5p. Biosci Biotechnol Biochem N/A:1-13 (2019). PubMed: 31791206
    • Bhargava R  et al. C-NHEJ without indels is robust and requires synergistic function of distinct XLF domains. Nat Commun 9:2484 (2018). PubMed: 29950655
    • Zhou Z  et al. siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice. Oncol Lett 11:2806-2814 (2016). IHC . PubMed: 27073556

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Great secondary antibody for Western Blot

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    I used this antibody to detect a human neuroprotein antibody (purchased from another vendor) and it worked the very first time at a dilution of 1:2000 and with an incubation time of 1h at room temperature. Highly recommended.

    Abcam user community

    Verified customer

    Submitted May 11 2020

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