Overview

  • Product name
    Donkey Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Goat
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/2500.

Images

  • IHC image of beta Actin staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8229,10ug/ml overnight at +4°C. An HRP-conjugated secondary (Ab6885, 1/250 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6885 was used at dilution 1/5000 with the primary antibody ab36146 in WB. See the review on ab36146.

References

This product has been referenced in:
  • Flores-Costa R  et al. The soluble guanylate cyclase stimulator IW-1973 prevents inflammation and fibrosis in experimental non-alcoholic steatohepatitis. Br J Pharmacol 175:953-967 (2018). Read more (PubMed: 29281143) »
  • Yu R  et al. miR-377 targets CUL4A and regulates metastatic capability in ovarian cancer. Int J Mol Med 41:3147-3156 (2018). Read more (PubMed: 29512715) »
See all 30 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting us.

As I presume these antibodies will be used in ICC/IF (which is the application ab13627 has been tested in), I would then suggest using ab6885 or ab6741 as secondary antibody.

Both should recognise and be suitable for detecting ab13627. If you had any problem using any of these, please note that all of our products are covered by the Abpromise guarantee, and in case they do not perform as stated on the datasheet we will replace or refund the product.

I’d like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMab free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Please do not hesitate to contact us for further assistance.

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Answer

I have requested that you be sent 3 new antibodies for the customer and I have also asked that the vials be checked for brown precipitate before being shipped.

Thank you for all your help with this matter and I hope it all works out.

Please let me know if there is anything else I can help you with.

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Answer

I am really sorry that the customer is so upset, that was never my intention. I understand that they are well versed in the WB field; howeverI was only trying to get some more information on the protocol that they had used.

If the customers are feeling this strongly about the issue, I am happy to fully refund the cost of their purchase, or if there are prepared to give us another chance, then I can send replacements.

Please let me know whether you would like to me to send replacements, if so, which ones, or if the customer would like a refund.

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Answer

Thank you for contacting Abcam.

I am sorry that your customer is still having problems with these antibodies. As I mentioned in my previous email in November, could you provide me with the protocol that they are using and I can see if there is any information that I can give that will help.

Do the primary antibodies that they are using work when they use them with different secondary antibodies (not the ones they are having issues with?).

The antibodiesare covered under our Abpromise for six months and is guaranteed to work in western blot . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund. However, we do need to have some description of the problem before we can issue you any replacement or a refund.

I look forward to your response and resolving this issue.

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Answer

I am sorry that you are experiencing problems with the HRP tagged antibodies that you purchased from us. As you mentioned in the email that was sent, it is common for hrp tagged secondaries to have a brown precipitate in the vial, but vortexing should allow the antibody to work as stated. I was wondering if you could give me some more information on how you tested the antibodies, application, incubation times, concentration of antibody used. Also, there is a way to test the reactivity of hrp tagged secondaries. Take 500ul of your mixed ECL reagent into the dark room and then add 1ul of your secondary antibody into the tube. If the secondary antibody is active then the solution will turn a fluorescent blue. Have you tried this with the antibodies? Unfortunately the order number that was used to purchase these products did not come I look forward to you reply and helping you resolve this issue.

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Answer

We recommend the follwing dilutions: ELISA 1:4,000 - 1:10,000 Immunoblotting 1:2,000 - 1:5,000 Immunohistochemistry 1:200 - 1:2,000

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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