Donkey Anti-Goat IgG H&L (HRP) (ab6885)

Submit a review Q&A (6)References (36)

Overview

  • Product name
    Donkey Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Goat
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/2500.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)Banerjee et al PLoS One. 2013 Nov 15;8(11):e80940. doi: 10.1371/journal.pone.0080940. eCollection 2013. Fig 2.

    Immunohistologic images of expression of endometrial PECAM-1/CD31 in (Panel A) controls and (Panel B) IRSM (Idiopathic recurrent spontaneous miscarriage).

    3-5 µm thick sections obtained from formaldehyde fixed, paraffin-embedded human tissue were dehydrated in graded ethanol. After antigen retrieval, slides were incubated for an hour in 3% blocking serum (BSA) in PBS for controlling non-specific binding of primary antibody. The slides were then incubated with goat anti-PECAM 1. Excess primary antibody was washed with PBS and the sections were again incubated with ab6885.

    Labeled cells were visualized with diaminobenzidine (DAB) and sections counterstained with hematoxylin. Next, the slides were dehydrated using series of alcohol gradient and mounted using distrene, tricresyl phosphate (DPX) and xylene.

  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
    Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)Mavroconstanti et al PLoS One. 2013 Aug 1;8(8):e71445. doi: 10.1371/journal.pone.0071445. Print 2013. Fig S1.

    Expression levels of wild type and variant GFP-CDH13 fusion proteins in HEK-293 cells.

    Panel A: GFP-CDH13 fusion proteins (26 kDa+105 kDa?=?131 kDa) were detected in HEK-293 cells by an antibody against GFP. Mock cells transfected with the empty GFP vector expressed only GFP (26 kDa).

    ab6885 used at a 1:5000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)

    IHC image of beta Actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229,10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6885, 1/250 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
    Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)

    ab6885 was used at dilution 1/5000 with the primary antibody ab36146 in WB. See the review on ab36146.

References

This product has been referenced in:
  • Yang X  et al. Benzene metabolite hydroquinone promotes DNA homologous recombination repair via the NF-?B pathway. Carcinogenesis N/A:N/A (2019). Read more (PubMed: 30770924) »
  • Flores-Costa R  et al. The soluble guanylate cyclase stimulator IW-1973 prevents inflammation and fibrosis in experimental non-alcoholic steatohepatitis. Br J Pharmacol 175:953-967 (2018). Read more (PubMed: 29281143) »
See all 36 Publications for this product

Publishing research using ab6885? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-6 of 6 Q&A

Question

Dear Sir/ Madam,
I am looking for a peroxidase secondary antibody to reacts with goat. The antibody that I am using is ab 13627. In your website I found ab 6741, ab 6885 and ab 97100. Could you please indicate which one is better to react with ab13627? Do you have any other suggestion?
Best regards,

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Answer

Thank you for contacting us.

As I presume these antibodies will be used in ICC/IF (which is the application ab13627 has been tested in), I would then suggest using ab6885 or ab6741 as secondary antibody.

Both should recognise and be suitable for detecting ab13627. If you had any problem using any of these, please note that all of our products are covered by the Abpromise guarantee, and in case they do not perform as stated on the datasheet we will replace or refund the product.

I’d like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMab free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Please do not hesitate to contact us for further assistance.

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Question

I convinced them to accept the replacements. I believe they will not have problems with the new secondaries, but please make sure the replacements do not have brown precipitates in the vials.

I am looking forward to hearing from you.

Best Regards,

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Answer

I have requested that you be sent 3 new antibodies for the customer and I have also asked that the vials be checked for brown precipitate before being shipped.

Thank you for all your help with this matter and I hope it all works out.

Please let me know if there is anything else I can help you with.

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Question

I have some bad news. Please, read below what customer answered me about this case:

I would like to inform you that the antibodies were tested in WB experiments and the incubation time and concentrations used are the same that work for other secondary antibodies from other brands and even from Abcam.

The test you suggested, to put 1ul secondary + 500 ul ECL, does not mean the secondary is recognizing the primary, but that the HRP is working.

So, I am sorry to inform you that we do not want to buy more from Sellex and I will then consider to purchase antibodies from other companies (Sigma, Ge, Millipore), as Abcam is not facilitating the exchange of so few antibodies and the laboratory can not lose these reagents.
I hope you send this information to Abcam, and replace the secondaries as fast as possible; we have already informed and testedeverything we could."


I am looking forward to hearing from you.

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Answer

I am really sorry that the customer is so upset, that was never my intention. I understand that they are well versed in the WB field; howeverI was only trying to get some more information on the protocol that they had used.

If the customers are feeling this strongly about the issue, I am happy to fully refund the cost of their purchase, or if there are prepared to give us another chance, then I can send replacements.

Please let me know whether you would like to me to send replacements, if so, which ones, or if the customer would like a refund.

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Question

\Our customer is still complaining that all these 3 secondaries are not working. The group has experience inWBapplication as this is the major techniqueperformed in their lab.Unfortunatelly, they are very disapointed with these secondaries they received.

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Answer

Thank you for contacting Abcam.

I am sorry that your customer is still having problems with these antibodies. As I mentioned in my previous email in November, could you provide me with the protocol that they are using and I can see if there is any information that I can give that will help.

Do the primary antibodies that they are using work when they use them with different secondary antibodies (not the ones they are having issues with?).

The antibodiesare covered under our Abpromise for six months and is guaranteed to work in western blot . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund. However, we do need to have some description of the problem before we can issue you any replacement or a refund.

I look forward to your response and resolving this issue.

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Question

For the technical support,   Customer has reported brown precipitates in the vials below, and after testing none of them worked. I suggested vortexing the vials as recommended on the datasheet, but nothing changed, customer says the antibodies are not working.    ab6721 (2 units) lot GR27131-2 ab6789 (2 units) lot GR23382-2 ab6885 (1 unit) lot GR34440-1 "The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal."   I am providing the order number and I look forward to hearing from you.  

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Answer

I am sorry that you are experiencing problems with the HRP tagged antibodies that you purchased from us. As you mentioned in the email that was sent, it is common for hrp tagged secondaries to have a brown precipitate in the vial, but vortexing should allow the antibody to work as stated. I was wondering if you could give me some more information on how you tested the antibodies, application, incubation times, concentration of antibody used. Also, there is a way to test the reactivity of hrp tagged secondaries. Take 500ul of your mixed ECL reagent into the dark room and then add 1ul of your secondary antibody into the tube. If the secondary antibody is active then the solution will turn a fluorescent blue. Have you tried this with the antibodies? Unfortunately the order number that was used to purchase these products did not come I look forward to you reply and helping you resolve this issue.

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Question

Hej ! What's the optimal dilution for this ab ? (8. Dilute the horseradish peroxidase labeled secondary antibody at the optimal dilution (according to the manufacturer) in blocking buffer.)

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Answer

We recommend the follwing dilutions: ELISA 1:4,000 - 1:10,000 Immunoblotting 1:2,000 - 1:5,000 Immunohistochemistry 1:200 - 1:2,000

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