Question (28461) | Donkey Anti-Goat IgG H&L (HRP) (ab6885)

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Question

For the technical support,   Customer has reported brown precipitates in the vials below, and after testing none of them worked. I suggested vortexing the vials as recommended on the datasheet, but nothing changed, customer says the antibodies are not working.    ab6721 (2 units) lot GR27131-2 ab6789 (2 units) lot GR23382-2 ab6885 (1 unit) lot GR34440-1 "The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal."   I am providing the order number and I look forward to hearing from you.  

Answer

I am sorry that you are experiencing problems with the HRP tagged antibodies that you purchased from us. As you mentioned in the email that was sent, it is common for hrp tagged secondaries to have a brown precipitate in the vial, but vortexing should allow the antibody to work as stated. I was wondering if you could give me some more information on how you tested the antibodies, application, incubation times, concentration of antibody used. Also, there is a way to test the reactivity of hrp tagged secondaries. Take 500ul of your mixed ECL reagent into the dark room and then add 1ul of your secondary antibody into the tube. If the secondary antibody is active then the solution will turn a fluorescent blue. Have you tried this with the antibodies? Unfortunately the order number that was used to purchase these products did not come I look forward to you reply and helping you resolve this issue.

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