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I have some bad news. Please, read below what customer answered me about this case:
I would like to inform you that the antibodies were tested in WB experiments and the incubation time and concentrations used are the same that work for other secondary antibodies from other brands and even from Abcam.
The test you suggested, to put 1ul secondary + 500 ul ECL, does not mean the secondary is recognizing the primary, but that the HRP is working.
So, I am sorry to inform you that we do not want to buy more from Sellex and I will then consider to purchase antibodies from other companies (Sigma, Ge, Millipore), as Abcam is not facilitating the exchange of so few antibodies and the laboratory can not lose these reagents.
I hope you send this information to Abcam, and replace the secondaries as fast as possible; we have already informed and testedeverything we could."
I am looking forward to hearing from you.
Asked on Jan 31 2012
I am really sorry that the customer is so upset, that was never my intention. I understand that they are well versed in the WB field; howeverI was only trying to get some more information on the protocol that they had used.
If the customers are feeling this strongly about the issue, I am happy to fully refund the cost of their purchase, or if there are prepared to give us another chance, then I can send replacements.
Please let me know whether you would like to me to send replacements, if so, which ones, or if the customer would like a refund.
Answered on Jan 31 2012