Overview

  • Product name
    Donkey Anti-Goat IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Goat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, ICCmore details
  • Immunogen

    Other Immunogen Type corresponding to Goat IgG. Contains both the heavy and light chain

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.05% Proclin
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC 1/200 - 1/500.

Images

  • IHC image of beta actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97110, 1/100 dilution) was used for 1 hour at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/200 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 & 5-6 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/20000 dilution
    Lanes 3-4 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20 minutes

References

This product has been referenced in:
  • Schepens B  et al. Vaccination with influenza hemagglutinin-loaded ceramic nanoporous microneedle arrays induces protective immune responses. Eur J Pharm Biopharm 136:259-266 (2019). Read more (PubMed: 30731115) »
  • Zhu Z  et al. Gene Expression and Protein Synthesis in Mitochondria Enhance the Duration of High-Speed Linear Motility in Boar Sperm. Front Physiol 10:252 (2019). Read more (PubMed: 30914972) »
See all 25 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Abreviews
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab166930 (1:2000).

Mr. Baptiste Jude

Verified customer

Submitted Jul 06 2016

Abreviews
Application
Western blot
This antibody is good for western blot assay, a dilution of 1 to 5,000 could always offer a good detection signal, which was comparable to the dilution of 1 to 2,000.

Abcam user community

Verified customer

Submitted Nov 05 2015

Abreviews
Application
Western blot
a good 2nd Ab for WB (1:5,000 dilution).

Abcam user community

Verified customer

Submitted Nov 02 2015

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