Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

Submit a review Q&A (2)References (8)
Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

    Key features and details

    • Donkey Anti-Goat IgG H&L (HRP) preadsorbed
    • Conjugation: HRP
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF

    Overview

    • Product name

      Donkey Anti-Goat IgG H&L (HRP) preadsorbed
      See all IgG secondary antibodies

    • Host species

      Donkey

    • Target species

      Goat

    • Tested applications

      Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details

    • Minimal
      cross-reactivity


      Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

    • Immunogen

      Full length native Goat IgG (purified).

    • Conjugation

      HRP

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.50
      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • Conjugation notes

      Horseradish Peroxidase (HRP)

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab7125 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    1/170000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/2000 - 1/10000.
    ICC/IF
    1/500 - 1/2500.
    Notes
    Dot blot
    Use at an assay dependent dilution.
    ELISA
    1/170000.
    IHC-P
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    Immunomicroscopy
    Use at an assay dependent dilution.
    WB
    1/2000 - 1/10000.
    ICC/IF
    1/500 - 1/2500.

    Images

    • Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)
      Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)
      Ab7125 was used at dilution 1/10000 with the primary antibody ab37917 in WB. See the review on ab37917.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (8)

    Publishing research using ab7125? Please let us know so that we can cite the reference in this datasheet.

    ab7125 has been referenced in 8 publications.

    • Li L  et al. Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy. Exp Ther Med 18:4313-4321 (2019). PubMed: 31777538
    • Nassar M  et al. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa. Proc Natl Acad Sci U S A 114:E337-E346 (2017). PubMed: 28049839
    • Rinaldi L  et al. Mitochondrial AKAP1 supports mTOR pathway and tumor growth. Cell Death Dis 8:e2842 (2017). IHC . PubMed: 28569781
    • Jendresen C  et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). PubMed: 28320424
    • Du Roure C  et al. Hematopoietic Overexpression of FOG1 Does Not Affect B-Cells but Reduces the Number of Circulating Eosinophils. PLoS One 9:e92836 (2014). WB . PubMed: 24747299
    • Mornet E  et al. Folate-equipped nanolipoplexes mediated efficient gene transfer into human epithelial cells. Int J Mol Sci 14:1477-501 (2013). PubMed: 23344053
    • Maekawa T  et al. Coiled-Coil Domain-Dependent Homodimerization of Intracellular Barley Immune Receptors Defines a Minimal Functional Module for Triggering Cell Death. Cell Host Microbe 9:187-99 (2011). WB . PubMed: 21402358
    • Graham D  et al. Candidate genes that determine response to salt in the stroke-prone spontaneously hypertensive rat: congenic analysis. Hypertension 50:1134-41 (2007). WB . PubMed: 17938382

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Question

    I want to inquire about the presence of kits of elisa IGG OF cat
    or conjugated of cat , and kits of IFAT IGG OF CAT or conjugated of cat
    You are kindly requested to respond as soon as possible
    I would also like to know the agent for the supply of this order in the Kingdom of Saudi Arabia
    thanks for time....

    Read More
    Answer

    Thank you for contacting us.



    Our distributor in Saudi-Arabia:

    Abdulla Fouad Holding Co.
    Abdulla Fouad Head Quarter Bldg.
    Dammam-Khobar Highway
    P.O. Box 257, Dammam
    31411
    Saudi Arabia
    Tel. +96626394455
    Fax. +96626396725
    http://www.abdulla-fouad.com



    We do not have kits for the detection of cat IgG, butI found anantibodywhich may interest you:

    https://www.abcam.com/Goat-Cat-IgG-Fc-ab112799.html (or use the following: https://www.abcam.com/Goat-Cat-IgG-Fc-ab112799.html).

    This antibody has been tested in ELISA and can be used with a secondary antibody like ab7125 Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed, to be used in an ELISA experiment.

    https://www.abcam.com/Donkey-Goat-IgG-HL-HRP-preadsorbed-ab7125.html (or use the following: https://www.abcam.com/Donkey-Goat-IgG-HL-HRP-preadsorbed-ab7125.html).

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    Question

    I have various normal human cell lines and tumor cell lines. My primary antibody is raised in goat. I am getting bands showing up in the negative control (secondary only). Looks like the secondary is binding non-specifically to the proteins in the lysates. I am using it at a 1/2000 dilution. I had the same problem with another secondary from another company, but this problem was cleared up by using one that had been pre-asorbed against human IgG. Has this secondary antibody been pre-adsorbed against human IgG, also?

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    Answer

    Ab7125 has only been pre-adsorbed to the following: Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. So, it most likely is binding non-specifically to proteins in your lysates. If you have any more questions, please contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com