Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)
Key features and details
- Donkey Anti-Goat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF
Overview
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Product name
Donkey Anti-Goat IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details -
Minimal
cross-reactivity
Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
Immunogen
Full length native Goat IgG (purified).
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
Use at an assay dependent dilution.
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ELISA |
1/170000.
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IHC-P |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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Immunomicroscopy |
Use at an assay dependent dilution.
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WB |
1/2000 - 1/10000.
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ICC/IF |
1/500 - 1/2500.
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Notes |
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Dot blot
Use at an assay dependent dilution. |
ELISA
1/170000. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
WB
1/2000 - 1/10000. |
ICC/IF
1/500 - 1/2500. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (10)
ab7125 has been referenced in 10 publications.
- Liu G et al. CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia-induced damage. Mol Med Rep 23:N/A (2021). PubMed: 33537819
- Wu D et al. Ameliorating effect of quercetin on epilepsy by inhibition of inflammation in glial cells. Exp Ther Med 20:854-859 (2020). PubMed: 32742328
- Li L et al. Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy. Exp Ther Med 18:4313-4321 (2019). PubMed: 31777538
- Nassar M et al. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa. Proc Natl Acad Sci U S A 114:E337-E346 (2017). PubMed: 28049839
- Rinaldi L et al. Mitochondrial AKAP1 supports mTOR pathway and tumor growth. Cell Death Dis 8:e2842 (2017). IHC . PubMed: 28569781
- Jendresen C et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). PubMed: 28320424
- Du Roure C et al. Hematopoietic Overexpression of FOG1 Does Not Affect B-Cells but Reduces the Number of Circulating Eosinophils. PLoS One 9:e92836 (2014). WB . PubMed: 24747299
- Mornet E et al. Folate-equipped nanolipoplexes mediated efficient gene transfer into human epithelial cells. Int J Mol Sci 14:1477-501 (2013). PubMed: 23344053
- Maekawa T et al. Coiled-Coil Domain-Dependent Homodimerization of Intracellular Barley Immune Receptors Defines a Minimal Functional Module for Triggering Cell Death. Cell Host Microbe 9:187-99 (2011). WB . PubMed: 21402358
- Graham D et al. Candidate genes that determine response to salt in the stroke-prone spontaneously hypertensive rat: congenic analysis. Hypertension 50:1134-41 (2007). WB . PubMed: 17938382