Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

Submit a review Q&A (2)References (7)

Overview

  • Product name
    Donkey Anti-Goat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Goat
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • Minimal
    cross-reactivity

    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details
  • Immunogen

    Full length native Goat IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA 1/170000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ICC/IF 1/500 - 1/2500.

Images

  • Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)
    Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)
    Ab7125 was used at dilution 1/10000 with the primary antibody ab37917 in WB. See the review on ab37917.

References

This product has been referenced in:
  • Jendresen C  et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). Read more (PubMed: 28320424) »
  • Nassar M  et al. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa. Proc Natl Acad Sci U S A 114:E337-E346 (2017). Read more (PubMed: 28049839) »
See all 7 Publications for this product

Publishing research using ab7125? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I want to inquire about the presence of kits of elisa IGG OF cat
or conjugated of cat , and kits of IFAT IGG OF CAT or conjugated of cat
You are kindly requested to respond as soon as possible
I would also like to know the agent for the supply of this order in the Kingdom of Saudi Arabia
thanks for time....

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Answer

Thank you for contacting us.



Our distributor in Saudi-Arabia:

Abdulla Fouad Holding Co.
Abdulla Fouad Head Quarter Bldg.
Dammam-Khobar Highway
P.O. Box 257, Dammam
31411
Saudi Arabia
Tel. +96626394455
Fax. +96626396725
http://www.abdulla-fouad.com



We do not have kits for the detection of cat IgG, butI found anantibodywhich may interest you:

https://www.abcam.com/index.html?datasheet=112799 (or use the following: https://www.abcam.com/index.html?datasheet=112799).

This antibody has been tested in ELISA and can be used with a secondary antibody like ab7125 Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed, to be used in an ELISA experiment.

https://www.abcam.com/index.html?datasheet=7125 (or use the following: https://www.abcam.com/index.html?datasheet=7125).

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Question

I have various normal human cell lines and tumor cell lines. My primary antibody is raised in goat. I am getting bands showing up in the negative control (secondary only). Looks like the secondary is binding non-specifically to the proteins in the lysates. I am using it at a 1/2000 dilution. I had the same problem with another secondary from another company, but this problem was cleared up by using one that had been pre-asorbed against human IgG. Has this secondary antibody been pre-adsorbed against human IgG, also?

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Answer

Ab7125 has only been pre-adsorbed to the following: Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. So, it most likely is binding non-specifically to proteins in your lysates. If you have any more questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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