Product nameDonkey Anti-Goat IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies
DescriptionDonkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP), pre-adsorbed
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Chicken, Human, Mouse, Pig, Rabbit, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab97120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/200 - 1/5000.|
|ELISA||1/10000 - 1/100000. (Primary)|
|WB||1/5000 - 1/50000. Colorimetric 1/5000 - 1/30000; Chemiluminescent 1/10000 - 1/50000|
This product has been referenced in:
- Dominy SS et al. Porphyromonas gingivalis in Alzheimer's disease brains: Evidence for disease causation and treatment with small-molecule inhibitors. Sci Adv 5:eaau3333 (2019). Read more (PubMed: 30746447) »
- Pittala S et al. Targeting Liver Cancer and Associated Pathologies in Mice with a Mitochondrial VDAC1-Based Peptide. Neoplasia 20:594-609 (2018). IHC-P . Read more (PubMed: 29747160) »