Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed (ab7004)


  • Product name
    Donkey Anti-Goat IgG H&L (Phycoerythrin) preadsorbed
    See all IgG secondary antibodies
  • Host species
  • Target species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • Minimal

    Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat more details
  • Immunogen

    Goat IgG whole molecule

  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notes
    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality
  • Isotype
  • General notes

    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas


Our Abpromise guarantee covers the use of ab7004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications.
Flow Cyt 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.


This product has been referenced in:
  • Lan P  et al. TNF superfamily receptor OX40 triggers invariant NKT cell pyroptosis and liver injury. J Clin Invest 127:2222-2234 (2017). Read more (PubMed: 28436935) »
  • Rué L  et al. Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels. J Clin Invest 126:4319-4330 (2016). Read more (PubMed: 27721240) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Flow Cytometry
Very bright secondary antibody and PE is one of the best if you have a weak antigen. I used this in a perfect pairing with FITC in a FACS experiment to check pluripotency markers in human embryonic stem cells.

Abcam user community

Verified customer

Submitted Sep 28 2015


Thank you for your enquiry. No reaction was observed with ab7004 against ant-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, or Rat serum proteins or other goat heavy or light chain proteins. Pig was not tested. If you have any further questions, please contact us again.

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Thank you for your reply. Generally, if you intend to detect an intracellular protein via FACS analysis, you need to permeabilise the cells (i.e. using permeabilization buffer containing saponin) before the staining procedure. This secondary antibody should be suitable for recognizing both intracellular and extracellular epitopes. However, you must check the suitability of the primary antibody as well. Make sure that the primary and the secondary are compatible with each other and they both have been tested for FACS analysis.

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Thank you for your enquiry and your interest in our products. According to the datasheet, this secondary antibody (ab7004) has been tested for FACS/Flow cytometry. For details on general FACS analysis, please click on the “Protocols“ section on the right hand side panel of the Abcam homepage, you might find some useful information there.

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We do not have a concentration for the antibody as such. However, the concentration of the label is 0.5 mg/ml, and we would estimate the antibody to be about 1 mg/ml.

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