Donkey Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed (ab102422)

Overview

  • Product name

    Donkey Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Human
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Other Immunogen Type corresponding to IgG.

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.10
    Preservative: 0.09% Sodium azide
    Constituents: 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/1000 - 1/10000. As a Primary
WB 1/1000 - 1/10000. Chemiluminescent detection.

References

ab102422 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab102422.
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