Key features and details
- Donkey polyclonal Secondary Antibody to Human IgG - H&L (DyLight® 488), pre-adsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, Flow Cyt
Product nameDonkey Anti-Human IgG H&L (DyLight® 488) preadsorbed
See all IgG secondary antibodies
DescriptionDonkey polyclonal Secondary Antibody to Human IgG - H&L (DyLight® 488), pre-adsorbed
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
Tested applicationsSuitable for: ICC, IHC-P, Flow Cytmore details
Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
ConjugationDyLight® 488. Ex: 493nm, Em: 518nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Our Abpromise guarantee covers the use of ab102424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/50 - 1/500.|
|IHC-P||1/50 - 1/500.|
|Flow Cyt||1/50 - 1/200.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab102424 has not yet been referenced specifically in any publications.