Key features and details
- Donkey polyclonal Secondary Antibody to Human IgG - H&L, pre-adsorbed
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameDonkey Anti-Human IgG H&L preadsorbed
See all IgG secondary antibodies
DescriptionDonkey polyclonal Secondary Antibody to Human IgG - H&L, pre-adsorbed
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Other Immunogen Type corresponding to IgG.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab102421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/200 - 1/2000.|
|ELISA||1/100 - 1/30000. ELISA - Coating: 1/100 - 1/500; ELISA - Primary: 1/1000 - 1/30000|
|WB||1/1000 - 1/30000. WB - Colorimetric: 1/1000 - 1/10000; WB - Chemiluminescent: 1/1000 - 1/30000|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab102421 has been referenced in 1 publication.
- Henson D et al. Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report. Arterioscler Thromb Vasc Biol 39:1884-1892 (2019). PubMed: 31315438