Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (6nm Gold) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Mouse
  • Specificity

    Recognizes the heavy chains on mouse IgG and the light chains common to most murine immunoglobulins. Does not crossreact with non-immunoglobulin serum proteins. Donkey Anti-Mouse IgG H&L (6nm Gold) preadsorbed exhibits minimal cross reactivity to Bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit and sheep serum proteins.

  • Tested applications

    Suitable for: Electron Microscopy, WB, ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Sheep more details
  • Conjugation

    Gold 6nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 8.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.381% Sodium borate, 0.164% Sodium phosphate, 0.87% Sodium chloride, 1.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy 1/20 - 1/40.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

I have been in contact with the originator of these products. Unfortunately they do not have any direct experience doing these types of procedures with pre-conjugated products and cannot provide any further information.

My colleagues and I believe that these procedures should be effective for removal of unwanted buffer additives from the antibody however we would likely recommend using the antibody concentration kit as it seems less likely that damage may occur. Our concentration kits may be found at:

1 column:

https://www.abcam.com/Antibody-Concentration-Kit-1-column-ab102776.html

3 columns:

https://www.abcam.com/Antibody-Concentration-Kit-3-columns-ab102778.html

Please remember that these products are covered by our Abpromise guarantee. We are happy to provide scientific support, replacement or refund should a product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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