Donkey Anti-Mouse IgG H&L (ab182022)
Key features and details
- Donkey Anti-Mouse IgG H&L
- Host species: Donkey
- Isotype: IgG
- Suitable for: ELISA, IHC-P, WB, IP
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Specificity
The antibody reacts with mouse immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, rabbit IgG, goat IgG and Chicken IgY, less than 2%. Rat IgG, less than 33%. -
Tested applications
Suitable for: ELISA, IHC-P, WB, IPmore details -
Immunogen
The details of the immunogen for this antibody are not available.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/20000 - 1/200000.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Notes |
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ELISA
1/20000 - 1/200000. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Images
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Cross-reactivity of the polyclonal secondary antibody ab182022 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182022 was then added starting at 1 µg/ml and gradually diluted 1:4 (50µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1:20,000 dilution (50µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182022 showed a cross-reactivity below 2% towards all rabbit IgG, human IgG, goat IgG and chicken IgY and 33% towards rat IgG.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab182022 has been referenced in 1 publication.
- Hauptman D et al. Leydig Cells in Patients with Non-Obstructive Azoospermia: Do They Really Proliferate? Life (Basel) 11:N/A (2021). PubMed: 34833142