Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175658)
Key features and details
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISAmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
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| ICC/IF | (1) |
1/200 - 1/1000.
We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
| IHC-P |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
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ICC/IF
1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
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IHC-P
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175658)
ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175658 Alexa Fluor® 405 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (ab175658)Emmission spectra of the Alexa Fluor® conjugated secondary antibodies.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab175658 has been referenced in 19 publications.
- Garcia-Oscos F et al. Autism-linked gene FoxP1 selectively regulates the cultural transmission of learned vocalizations. Sci Adv 7:N/A (2021). PubMed: 33536209
- Nguyen NM et al. Three-Dimensional Reconstruction of Neurovascular Network in Whole Mount Preparations and Thick-Cut Transverse Sections of Mouse Urinary Bladder. World J Mens Health 39:131-138 (2021). PubMed: 31496149
- Kakihana T et al. The optineurin/TIA1 pathway inhibits aberrant stress granule formation and reduces ubiquitinated TDP-43. iScience 24:102733 (2021). PubMed: 34258561
- Bosakova M et al. Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signaling. EMBO Mol Med 12:e11739 (2020). PubMed: 33200460
- Han Q et al. Restoring Cellular Energetics Promotes Axonal Regeneration and Functional Recovery after Spinal Cord Injury. Cell Metab 31:623-641.e8 (2020). PubMed: 32130884