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    donkey-mouse-igg-hl-alexa-fluor-488-ab150105.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (187)

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
  • Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)

Key features and details

  • Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, IHC-P, ELISA, Flow Cyt

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, ELISA, Flow Cytmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • Alexa Fluor® 488

Associated products

  • Related Products

    • Normal Donkey Serum (ab7475)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF (2)
1/200 - 1/1000.
IHC-P (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/2000.

ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/2000.

ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

Images

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)

    ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150105 Alexa Fluor® 488 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
    Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (187)

Publishing research using ab150105? Please let us know so that we can cite the reference in this datasheet.

ab150105 has been referenced in 187 publications.

  • Kim J & Cooper JA Junctional Localization of Septin 2 Is Required for Organization of Junctional Proteins in Static Endothelial Monolayers. Arterioscler Thromb Vasc Biol 41:346-359 (2021). PubMed: 33147991
  • Bojanowski K  et al. Isosorbide Di-(Linoleate/Oleate) Stimulates Prodifferentiation Gene Expression to Restore the Epidermal Barrier and Improve Skin Hydration. J Invest Dermatol 141:1416-1427.e12 (2021). PubMed: 33181142
  • Gunjigake K  et al. Interleukin-17A derived from mast cells contributes to fibrosis in gastric cancer with peritoneal dissemination. Gastric Cancer 24:31-44 (2021). PubMed: 32488650
  • Hosoya M  et al. The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development. FEBS J 288:325-353 (2021). PubMed: 32323465
  • Vitiello PP  et al. Vulnerability to low-dose combination of irinotecan and niraparib in ATM-mutated colorectal cancer. J Exp Clin Cancer Res 40:15 (2021). PubMed: 33407715
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Detecting RUNX2 protein

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Worked very well against Mouse primary antibody against RUNX2
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 11 2018

Immunochemical staining of vimentin

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample: Human normal fibloblast cells
Treatment: 4% formalin fixation, 0.1% Triton-X100 permeabilization
1st antibody: anti-vimentin mouse monoclonal antibody
2nd antibody: anti-mouse IgG Alexa-488 (ab150105)
Mount: VECTASHIELD Mounting Medium with DAPI

Result: Specific staining with low background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 22 2017

Excellent - low background.

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Excellent antibody. Used at 1:500 dilution in PBS-Triton X-100 (0.1%) overnight at 4 degrees. Used in conjunction with GFAP antibody (Ab80842). Stable fluorescence when mounted with Vectashield (inc DAPI).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Craig Beall

Verified customer

Submitted Sep 08 2014

SALL4 staining in adult marmoset testis.

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This antibody works perfectly at a 1:500 dilution with SALL4 primary antibody (ab57577, mouse monoclonal). However, the intensity of this Donkey secondary antibody is weaker than that of the Goat secondary antibody (ab150113) at the same dilution. So that is why I gave it 4 stars.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. 煜欽 林

Verified customer

Submitted Oct 31 2013

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