Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150109)

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, ELISA, IHC-Pmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000.

ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Images

  • Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Donkey anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150109) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab150109 Alexa Fluor® 488 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Emmission spectra of the Alexa Fluor® conjugated secondary antibodies.

References

This product has been referenced in:

  • Weber JJ  et al. Calpastatin ablation aggravates the molecular phenotype in cell and animal models of Huntington disease. Neuropharmacology 133:94-106 (2018). ICC/IF . Read more (PubMed: 29355642) »
  • Jing Y  et al. Peptidoglycan Suppresses Phagocytic Activities and Apoptosis of Macrophages in Colonic Mucosa Tissues of Crohn's Disease Patients and In Vitro. Med Sci Monit 24:3382-3392 (2018). Read more (PubMed: 29787559) »
See all 18 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (PFA fixed)
Rat brain was perfusion-fixed using 4% PFA and cryo-sectioned in the coronal plane (20 µm thick).
Primary antibody: Mouse anti-NeuN antibody (Chemicon / Merck Millipore)
Secondary antibody: Donkey anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Abcam ab150109)
Blocking buffer: 10% Donkey serum, 3% BSA, 0.3% Triton X-100 in PBS (pH 7.4)
Brain section was blocked for 2 h at room temperature.
Incubation in primary antibody diluted in blocking buffer (1:400 dilution) at 4 °C for 16-18 h.
Incubation in secondary antibody diluted in PBS (1:2000 dilution) at room temperature for 2 h.
Image (scale bar = 200 µm) shown is taken from the cortical layer of the rat forebrain.
Strong fluorescent signal with moderate background noise can be observed.

Abcam user community

Verified customer

Submitted Nov 09 2016

Answer

We have not used this product with a LICOR system. Generally these systems use IR or fluorescent molecules such as Alexa Fluor 680, Alexa Fluor 750 for detection. We do not know if the system which you have will be able to excite and read the emission for an Alexa Fluor 488 fluorochrome. We suggest contacting your LICOR representative to determine if the system which you have will be able to do so.

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