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  1. Link

    donkey-mouse-igg-hl-alexa-fluor-647-ab150107.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (58)

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
  • Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
  • Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)

Key features and details

  • Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ELISAmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • Alexa Fluor® 647

Associated products

  • Related Products

    • Normal Donkey Serum (ab7475)
    • Anti-CD3 antibody [MEM-57] (ab8090)
    • Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
Flow Cyt
1/2000 - 1/4000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
1/2000 - 1/4000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.

Images

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)

    ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150107 Alexa Fluor® 647 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
    Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)

    Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Donkey anti-mouse IgG H&L (Alexa Fluor® 647) (ab150107) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.

  • Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)
    Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150107)

    Emmission spectra of the Alexa Fluor® conjugated secondary antibodies.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (58)

Publishing research using ab150107? Please let us know so that we can cite the reference in this datasheet.

ab150107 has been referenced in 58 publications.

  • Yoshida Y  et al. Alteration of circadian machinery in monocytes underlies chronic kidney disease-associated cardiac inflammation and fibrosis. Nat Commun 12:2783 (2021). PubMed: 33986294
  • Vukušic K  et al. Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Dev Cell 56:1253-1267.e10 (2021). PubMed: 33910056
  • Poon C  et al. Neutrophil recruitment and leukocyte response following focused ultrasound and microbubble mediated blood-brain barrier treatments. Theranostics 11:1655-1671 (2021). PubMed: 33408773
  • Hamm M  et al. BRN2 is a non-canonical melanoma tumor-suppressor. Nat Commun 12:3707 (2021). PubMed: 34140478
  • Danoy M  et al. Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment. APL Bioeng 5:026104 (2021). PubMed: 34027283
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Immunocytochemistry (primary airway epithelial cells)

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
1. Fixation: 95% ethanol for 10 min
2. Washing: 1x PBS for 3 min
3. Perm: PBS containing Triton X-100 for 10 min
4. 2M HCl for 8 min (60°C)
5. Antigen retrieval: 0.25% Trypsin (pH 7.8) for 30min (37°C)
6. Blocking: 1% BSA in PBST for 30 min
7. Primary antibody incubation (AB8152): 1% BSA in PBST for overnight (4°C)
8. Secondary antibody incubation: in 1% BSA in PBST 1 hour (room temp)
9. Mounting: Fluoroshield™
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Egi Kardia

Verified customer

Submitted Sep 10 2018

Glycogen synthase kinase-3 inhibition attenuates fibroblast activation and development of fibrosis following renal ischemia-reperfusion in mice.

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
I tried many different product to get secondary antibody for immunofluorescence staining better and more clear but failed then I bought abcam Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647)(ab150107) in kidney cells, as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best secondary antibody to detect immunofluorescence during in vivo studies
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Shailendra Singh

Verified customer

Submitted Aug 17 2018

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