Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680) (ab175774)

Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Mouse
  • Specificity
    This antibody is specific to Mouse IgG
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation
    Alexa Fluor® 680. Ex: 679nm, Em: 702nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • General notes

    We batch test Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680), ab175774 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680), ab175774 in these applications.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000.

Images

  • All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 5 µg
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 2 µg
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 1 µg

    Secondary
    All lanes : Donkey Anti-Mouse IgG H&L (Alexa Fluor® 680) (ab175774) at 1/10000 dilution

    Observed band size: 52 kDa (why is the actual band size different from the predicted?)



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175774 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

References

This product has been referenced in:
  • Lopez DH  et al. Tissue-selective alteration of ethanolamine plasmalogen metabolism in dedifferentiated colon mucosa. Biochim Biophys Acta 1863:928-938 (2018). WB . Read more (PubMed: 29709709) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
We used this secondary antibody for immunodetection of p53 on a LiCor Odyssee system (figure, part of this figure was used for the abreview of ab32132). The abcam ab175774 works good and gives a strong and intense signal. We did not observe any unspecific binding to the membrane. (Dilution 1:10000 in 5% milk in TBS-T buffer, nitrocellulose membrane).
Username

Mr. Christian Marx

Verified customer

Submitted Oct 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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