Product nameDonkey Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies
DescriptionDonkey polyclonal Secondary Antibody to Mouse IgG - H&L (AP)
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.10
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Conjugation notesMolar enzyme: antibody protein ratio is 1:1
Our Abpromise guarantee covers the use of ab97027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/5000 - 1/50000. (Primary)|
|WB||1/5000 - 1/50000. (Chemioluminescent)|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97027 has been referenced in 3 publications.
- Jezek J et al. Synergistic repression of thyroid hyperplasia by cyclin C and Pten. J Cell Sci 132:N/A (2019). PubMed: 31331961
- Jezek J et al. Mitochondrial translocation of cyclin C stimulates intrinsic apoptosis through Bax recruitment. EMBO Rep 20:e47425 (2019). PubMed: 31385392
- Ou L et al. High-dose enzyme replacement therapy in murine Hurler syndrome. Mol Genet Metab N/A:N/A (2013). PubMed: 24100243