Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7060)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/50000 - 1/200000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab7060? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

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    Dear Tracy, thank you for your information. We are interested in your products, in case you can provide the European Certificate( Country of Origin from Europe). As primary antibodies we choose ab2777 Prion protein PrP antibody [F 89/160.1.5]. An...

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    Unfortunatley we do not have country of origin certificates for our products nor do we offer six months warranty. Our guarentee is that an antibody should work as stated on the data sheet or we will refund or replace it. I also cannot hazard a guess at...

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    Hi, was this antibody adsorbed against Armenian hamster or Syrian Hamster or both? I am look it use it as a secondary in Western Blotting after immunoprecip. with an Armenian Hamster monoclonal and blotting with a polyclonal mouse serum. However, my ...

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    This antibody is pre-adsorbed against Golden Syrian Hamster serum proteins. The cross reactivity to this species is very minimized. Cross-reactivity to Armenian hamster is unknown.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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