Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)

Submit a review Q&A (3)References (14)
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
  • Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)

Key features and details

  • Donkey Anti-Mouse IgG H&L (DyLight® 488)
  • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, Flow Cyt

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP IRDye® 680RD IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (DyLight® 488)
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cytmore details

  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.
Flow Cyt
1/50 - 1/200.
Notes
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.
Flow Cyt
1/50 - 1/200.

Images

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)This image is courtesy of an Abreview submitted by Charles Pallangyo.

    ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.

    See Abreview

  • Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
    Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (14)

Publishing research using ab96875? Please let us know so that we can cite the reference in this datasheet.

ab96875 has been referenced in 14 publications.

  • Dolgodilina E  et al. Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance. Fluids Barriers CNS 17:17 (2020). PubMed: 32046769
  • Jara C  et al. Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue. Diabetol Metab Syndr 12:66 (2020). PubMed: 32774470
  • Sahasrabudhe A  et al. Cyfip1 Regulates SynGAP1 at Hippocampal Synapses. Front Synaptic Neurosci 12:581714 (2020). PubMed: 33613257
  • Li N  et al. Characterization of spontaneous spheroids from oral mucosa-derived cells and their direct comparison with spheroids from skin-derived cells. Stem Cell Res Ther 10:184 (2019). PubMed: 31234925
  • Lv K  et al. Incorporation of small extracellular vesicles in sodium alginate hydrogel as a novel therapeutic strategy for myocardial infarction. Theranostics 9:7403-7416 (2019). PubMed: 31695776
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Dear xxxxxx,

Below is the reply from my customer:

I wish to use the antibodies in a co-localization experiment. We have primary antibodies against specific Plasmodium falciparum proteins which localize to two different parasite organelles. The fluorescent secondary antibodies will then allow these organelles to be visualized.

We have not performed an experiment like this in the lab before and we will have to develop a protocol. This is the reason why I was wondering what the recommended protocol would be for the DyLight secondary antibodies.

I'm on a very strict budget and I'm looking for the most cost effective way to perform this experiment. In comparison, the Alexa Fluor secondary antibodies have a working concentration of 1ug/ml, with a supplied concentration of 2mg/ml. Do the DyLight secondary antibodies, with a supplied concentration of 0.5mg/ml, have a lower working concentration than that of the Alexa Fluor?

Thank you for your help

Regards

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Answer

Thank you for explaining this for me.

The antibodies mentioned, ab96875,ab96871,ab96877 andab96873 are all recommended to be used in a range of 1/50-1/500 for ICC and IF applications. This is only a starting point and optimisation of these conditions will be required by the end used and their particular experimental conditions. This will be the case with any secondary antibody. This equates to a final concentration of 1 to 10 ug/mL.

How many secondary antibodies are you looking to purchase? It may be that I can arrange for a discount for you?

I hope this information has been of help. I look forward to receiving your reply.

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Question

Dear tech Support

I have another query from a customer. I did send her the data sheets and concentration supplied. On the data sheet the suggested dilution is given, but she still has the following question. Please help. The antibodies in question:
AB96875
AB96871
AB96877
AB96873

Thank you for all your help

Regards

I need to know the concentration of antibody needed per reaction? So what volume of the given stock would I need to use in one experiment?
Best regards

Read More
Answer

Thank you for contacting us and your interest in our products.

The volume of antibody required per experiment would depend on what experiment the customer is hoping to perform, and what volume of diluted antibody the customer usually used in these experiments.

If the customer could please answer the following questions I can help in their request:

1. What application are you hoping to use these antibodies in?

2. Using your protocol, what volume of diluted antibody do you usually apply?

I look forward to receiving your reply.

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Question

Dear Sir/ Madam,

I wanna to study the N-cadherin for A7R5 (one kind of Mouse smooth muscle cell) and currentlyI have ordered the ab98952 but for the online sheet there is no 'secondary antibodies' tab on this product. May I know what kind of secondary antibody with FITC could be suitable for ab98952, for example, isGoat anti-Mouse IgG1 heavy chain (FITC) secondary antibody (a b97239) suitable? And I also wanna to know is there any conjugated antibody (conjugated withfluorescent secondary antibody) for N-cadherin? How about productAnti-N Cadherin antibody [GC-4], please recommend some for me, thanks a lot.

I am looking forward to your reply, and I hope I can order the right product ASAP.

Thanks a lot.

Best wishes,

Read More
Answer

Thank you for your enquiry and interest.

You could certainly use our advanced search engine specifically designed for secondary antibodies to find the most suitable products for your studies:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918



Since the isotype of this primary antibody is mouse IgG1, so you could select the isotype, target species, host species, format, specificity, conjugate using the drop-down menu.

We have different conjugates such as FITC, DyLight488, Chromeo 488, and here are a few suggestions:

FITC:

ab98692

ab97239

ab11588

You can find some further information about the the generation of our fluorochromes at this site:https://www.abcam.com/index.html?pageconfig=resource&rid=12948

DyLight488:

Affinity purified: ab96875, ab97015, ab96871

Pre-adsorbed: ab96879

Chromeo 488:

ab60313



If you need any further assistance in the future, please do not hesitate to contact me.

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