Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)

Submit an Abreview Q&A (3)Specific References (5)

Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)This image is courtesy of an Abreview submitted by Charles Pallangyo.

    ab7817 staining alpha smooth muscle actin in mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 5% BSA) for 2 hour at 25°C. A DyLight® 488-conjugated donkey anti-mouse IgG H&L (ab96875) (1/1000) was used as the secondary antibody. Vimentin appears in red.

    See Abreview

  • Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
    Donkey Anti-Mouse IgG H&L (DyLight® 488) (ab96875)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Wu J  et al. Stromal-epithelial lactate shuttle induced by tumor-derived interleukin-1ß promotes cell proliferation in oral squamous cell carcinoma. Int J Mol Med 41:687-696 (2018). IF . Read more (PubMed: 29207019) »
  • Dhanani KCH  et al. Fibronectin is a stress responsive gene regulated by HSF1 in response to geldanamycin. Sci Rep 7:17617 (2017). Read more (PubMed: 29247221) »

See all 5 Publications for this product

Publishing research using ab96875? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

Dear xxxxxx,

Below is the reply from my customer:

I wish to use the antibodies in a co-localization experiment. We have primary antibodies against specific Plasmodium falciparum proteins which localize to two different parasite or...

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Thank you for explaining this for me.

The antibodies mentioned, ab96875,ab96871,ab96877 andab96873 are all recommended to be used in a range of 1/50-1/500 for ICC and IF applications. This is only a starting point and optimisation of these co...

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Dear tech Support

I have another query from a customer. I did send her the data sheets and concentration supplied. On the data sheet the suggested dilution is given, but she still has the following question. Please help. The antibodies in qu...

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Thank you for contacting us and your interest in our products.

The volume of antibody required per experiment would depend on what experiment the customer is hoping to perform, and what volume of diluted antibody the customer usually used in ...

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Dear Sir/ Madam,

I wanna to study the N-cadherin for A7R5 (one kind of Mouse smooth muscle cell) and currentlyI have ordered the ab98952 but for the online sheet there is no 'secondary antibodies' tab on this product. May I know what kind of...

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Thank you for your enquiry and interest.

You could certainly use our advanced search engine specifically designed for secondary antibodies to find the most suitable products for your studies:

https://www.abcam.com/index.html?pagecon...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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