Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794)

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Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794)

    Key features and details

    • Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed
    • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: Flow Cyt, ICC/IF, IHC-P

    Overview

    • Product name

      Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed
      See all IgG secondary antibodies

    • Description

      Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed

    • Host species

      Donkey

    • Target species

      Mouse

    • Tested applications

      Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details

    • Minimal
      cross-reactivity


      Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details

    • Conjugation

      DyLight® 488. Ex: 493nm, Em: 518nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 0.2% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Associated products

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab98794 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Flow Cyt
      1/50 - 1/200.
      ICC/IF
      1/50 - 1/500.
      IHC-P
      1/50 - 1/500.
      Notes
      Flow Cyt
      1/50 - 1/200.
      ICC/IF
      1/50 - 1/500.
      IHC-P
      1/50 - 1/500.

      Images

      • Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794)
        Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794)
        Emission spectra of DyLight® fluorochromes available in our catalog.
        Line colors represent the approximate visible colors of the wavelength maxima.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (2)

      Publishing research using ab98794? Please let us know so that we can cite the reference in this datasheet.

      ab98794 has been referenced in 2 publications.

      • Shir A  et al. S101, an Inhibitor of Proliferating T Cells, Rescues Mice From Superantigen-Induced Shock. J Infect Dis 217:288-297 (2018). PubMed: 29149330
      • Gunness P  et al. 3D organotypic cultures of human HepaRG cells: a tool for in vitro toxicity studies. Toxicol Sci 133:67-78 (2013). PubMed: 23377618

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-5 of 5 Abreviews or Q&A

      Question

      Here isPO 310-61664B

      Best regards,

      Read More
      Answer

      Thank you for your email.

      The PO-310-61664B was ordered in October 2011 and the complaint was raised in July 2012; this means the product is out of Abpromise guarantee, which is 6 months from date of purchase.

      We have sold many units of these products without receiving any quality complaint so I believe that the problem might be with storage. Were these stored in dark; if not, then conjugates might have been damaged due to photo bleaching. Could you let me know how the products were stored?

      Thank you very much for your cooperation. I will look forward to hearing from you soon.

      Read More

      Question

      Hello
      Our customer said that primary antibodies are displayed at images (attached again in this email).
      In addition, her primary antibodies are raised on goat, so she can not
      use Alexa.
      Antibodies were stored as recommended (4oC).
      Order number - PI-004-61517-br
      Looking foward for a answer,
      Best regards,

      Read More
      Answer

      Could you please check the purchase order number; the number I-004-61517-br or 004-61517 provided does not match in our system? Could you also provide the purchase date of the product?
      I will look forward to hearing from you soon.

      Read More

      Question

      Attached below are the questionnaires of our customer.
      She is having problems with their secundary antibodies.
      Best regards,

      Read More
      Answer

      Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

      I have read the details you have kindly provided and have following further questions for better understanding of the problem;

      Could you provide the species of all primary antibodies used?
      Did you same experimental procedure with Alexa Fluor antibodies?
      Have you checked the Green and Redfilters
      Could you provide the order number? Howe the antibodies were stored?

      I will be looking forward to hearing from you soon.

      Read More

      Question

      I hope you are doing fine.



      Here are the ordering details:



      Po: 88908

      Lot: GR37446

      Invoice: 1321183



      The customer would like to receive a credit. Please issue the credit note.



      Thanks a lot for your assistance.

      Kind Regards,

      Read More
      Answer

      Thank you for contacting us.
      Your credit note ID is 21845.
      I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
      (1) Redeemed against the original invoice if this hasn't already been paid.
      (2) Held on the account for use against a future order.
      (3) A full refund can be offered where no other invoices are outstanding.
      Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
      To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
      The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
      I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

      Read More

      Question

      Dear Tech Support Team,

      Please read below the details of the customer's complaint and advise.

      Thanks in advance for your reply.


      Order Details: Donkey anti-Mouse IgG H&L (DyLight® 488) secondary antibody
      Antibody code: ab98794

      General Information:
      Antibody storage conditions +4oC
      Description of the problem non-specific staining
      Sample: Brain of rats
      Fixation of sample Methanol
      Antigen retrieval (Enzymatic method, Heat mediated technique etc.)
      Permeabilization step
      Blocking conditions PBS+8% normal donkey serum at 23oC for 60 min
      Primary Antibody Dilution between 1:100-1:500 on PBS+ 1.3% NDS over night at 4oC
      Secondary Antibody Donkey anti-Mouse IgG H&L (DyLight® 488) diluted 1:500-1:1000 in PBS+NDS incubation at RT for 120 min
      Detection method
      Positive and negative controls used We are working on frozen sections and we have tried double immunostaining with another anti rabbit secondary and we got excellent results with the anti rabbit and no signal with the Donkey anti-Mouse secondary. We also run this secondary antibody in another set of sections with a primary antibody that we know for certain that works. In one set the GR primary was run with the donkey anti mouse and in the second with goat anti mouse-Dylight -594 (from KPL). We got no results with the donkey-anti mouse and good results with the goat anti-mouse.
      Optimization attempts (problem solving):
      How many times have you tried the IHC? Several times with different primary antibody dilutions
      Have you run a "No Primary" control?
      Do you obtain the same results every time? Yes there is no signal



      Best Regards,

      Read More
      Answer

      Thank you for your enquiry regarding ab98794 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
      It is clear that the protocol worked fine and an alternative secondary antibody provided the expected results. The image you have kindly sent to me showed the significant difference in the staining pattern/quality.
      Could you please confirm the batch and order number. Would your troubled customer prefer to get a new vial (free of charge replacement) or a credit note?
      I look forward to hearing from you soon.

      Read More

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