Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab98794)

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Gunness P  et al. 3D organotypic cultures of human HepaRG cells: a tool for in vitro toxicity studies. Toxicol Sci 133:67-78 (2013). Read more (PubMed: 23377618) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Thank you for your email.

The PO-310-61664B was ordered in October 2011 and the complaint was raised in July 2012; this means the product is out of Abpromise guarantee, which is 6 months from date of purchase.

We have sold many units of these products without receiving any quality complaint so I believe that the problem might be with storage. Were these stored in dark; if not, then conjugates might have been damaged due to photo bleaching. Could you let me know how the products were stored?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Read More

Answer

Could you please check the purchase order number; the number I-004-61517-br or 004-61517 provided does not match in our system? Could you also provide the purchase date of the product?
I will look forward to hearing from you soon.

Read More

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

Could you provide the species of all primary antibodies used?
Did you same experimental procedure with Alexa Fluor antibodies?
Have you checked the Green and Redfilters
Could you provide the order number? Howe the antibodies were stored?

I will be looking forward to hearing from you soon.

Read More

Answer

Thank you for contacting us.
Your credit note ID is 21845.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Question

Dear Tech Support Team,

Please read below the details of the customer's complaint and advise.

Thanks in advance for your reply.


Order Details: Donkey anti-Mouse IgG H&L (DyLight® 488) secondary antibody
Antibody code: ab98794

General Information:
Antibody storage conditions +4oC
Description of the problem non-specific staining
Sample: Brain of rats
Fixation of sample Methanol
Antigen retrieval (Enzymatic method, Heat mediated technique etc.)
Permeabilization step
Blocking conditions PBS+8% normal donkey serum at 23oC for 60 min
Primary Antibody Dilution between 1:100-1:500 on PBS+ 1.3% NDS over night at 4oC
Secondary Antibody Donkey anti-Mouse IgG H&L (DyLight® 488) diluted 1:500-1:1000 in PBS+NDS incubation at RT for 120 min
Detection method
Positive and negative controls used We are working on frozen sections and we have tried double immunostaining with another anti rabbit secondary and we got excellent results with the anti rabbit and no signal with the Donkey anti-Mouse secondary. We also run this secondary antibody in another set of sections with a primary antibody that we know for certain that works. In one set the GR primary was run with the donkey anti mouse and in the second with goat anti mouse-Dylight -594 (from KPL). We got no results with the donkey-anti mouse and good results with the goat anti-mouse.
Optimization attempts (problem solving):
How many times have you tried the IHC? Several times with different primary antibody dilutions
Have you run a "No Primary" control?
Do you obtain the same results every time? Yes there is no signal



Best Regards,

Read More
Answer

Thank you for your enquiry regarding ab98794 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
It is clear that the protocol worked fine and an alternative secondary antibody provided the expected results. The image you have kindly sent to me showed the significant difference in the staining pattern/quality.
Could you please confirm the batch and order number. Would your troubled customer prefer to get a new vial (free of charge replacement) or a credit note?
I look forward to hearing from you soon.

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