For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    donkey-mouse-igg-hl-dylight-550-ab96876.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgG
Share by email

Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (13)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
  • Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
  • Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

Key features and details

  • Donkey Anti-Mouse IgG H&L (DyLight® 550)
  • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP PE Texas Red ® TRITC Unconjugated Unconjugated

You may also be interested in

Mounting
Product image
Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield (ab104135)
Blocking
Product image
Normal Donkey Serum (ab7475)

View more associated products

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (DyLight® 550)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Mouse
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 550 replaces DyLight® 549.
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Fluorophore
    • DyLight® 550

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550 – this information however will have no impact on your experiments. For more information please refer to DyLight® 550.

ICC/IF
1/50 - 1/500.
Flow Cyt
1/50 - 1/200.
WB
Use at an assay dependent concentration.
Notes
IHC-P
1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550 – this information however will have no impact on your experiments. For more information please refer to DyLight® 550.

ICC/IF
1/50 - 1/500.
Flow Cyt
1/50 - 1/200.
WB
Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

    Formalin-fixed paraffin-embedded sections of human ovarian carcinoma stained for p53 using a mouse anti-p53 (DO-1) antibody, followed by ab96876 at a 1:100 dilution (5 µg/ml). Counterstained with DAPI (blue).

  • Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)Courtesy of an anonymous Abreview
    All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution
    1. Lane 1 : Rat Hippocampus
    2. Lane 2 : Rat Hippocampus
    3. Lane 3 : Rat Hippocampus

    Lysates/proteins at 25 µg per lane.

    Secondary:

    1. Lane 1 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/5000 dilution
    2. Lane 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/1000 dilution
    3. Lane 3 : Other Commercial Dye at 1/1000 dilution
    4. Performed under reducing conditions.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (13)

Publishing research using ab96876? Please let us know so that we can cite the reference in this datasheet.

ab96876 has been referenced in 13 publications.

  • Bos R  et al. Altered action potential waveform and shorter axonal initial segment in hiPSC-derived motor neurons with mutations in VRK1. Neurobiol Dis 164:105609 (2022). PubMed: 34990802
  • Haydn T  et al. Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells. Int J Cancer 146:3435-3449 (2020). PubMed: 31755110
  • Zhang I  et al. Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures. Cells 9:N/A (2020). PubMed: 33171886
  • El-Bazzal L  et al. Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1. Hum Mol Genet 28:2378-2394 (2019). PubMed: 31090908
  • Kinslechner K  et al. Loss of SR-BI Down-Regulates MITF and Suppresses Extracellular Vesicle Release in Human Melanoma. Int J Mol Sci 20:N/A (2019). PubMed: 30823658
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Customer inquired about detecting presence of 7 different proteins in brain tissue via IHC-F. Would like 3-4 different host species for primary, secondary antibodies that do not overlap in spectra. Customer is also not sure if Caspase 3, Nogo, and neurofilament are necessary for detecting apoptosis / neuronal response.

Read More

Abcam community

Verified customer

Asked on Nov 27 2012

Answer

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numerous specific published references. The list for these 4 antibodies is given below:
1. GFAP ab4674 (https://www.abcam.com/gfap-antibody-astrocyte-marker-ab4674.html)
2. FOX3 ab104225 (https://www.abcam.com/fox3neun-antibody-ab104225.html)
3. Iba1 ab5076 (https://www.abcam.com/iba1-antibody-ab5076.html)
4. CD68 ab31630 (https://www.abcam.com/cd68-antibody-ed1-ab31630.html)

I also compiled anti-chicken, rabbit, goat, and mouse secondaries with separate emission spectra. We don't have secondaries for these species that weren't raised in any of the 4 species conjugated to a fluorescent protein with a similar spectrum to DAPI. Thus, I think your best bet is to obtain a far-red filter (Cy5), that way you could still stain with DAPI as well. The donkey secondaries that I found with separate emission spectra are listed below:
1. anti-chicken: FITC (https://www.abcam.com/chicken-igy-secondary-antibody-ab63507.html)
2. anti-rabbit: DyLight 594 (Similar spectrum to Texas Red) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-rabbit-igg-h-l-dylight-594-pre-adsorbed-ab96921.html)
3. anti-goat: DyLight 650 (Similar spectrum to Cy5) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-goat-igg-h-l-dylight-650-pre-adsorbed-ab96938.html)
4. anti-mouse: DyLight 550 (similar spectrum to TRITC) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-mouse-igg-h-l-dylight-550-ab96876.html)

The other antibodies you chose (Nogo, heavy neurofilament, caspase 3) seem to be good choices as well as they are tested in formaldehyde fixed, frozen sections via IHC in rat. Below are the antibodies we discussed against those three proteins:
1. anti-Nogo (https://www.abcam.com/nogo-antibody-ab32298.html)
2. anti-heavy neurofilament (https://www.abcam.com/200-kd-neurofilament-heavy-antibody-ab134459.html)
3. anti-caspase 3 (https://www.abcam.com/active-caspase-3-antibody-ab13847.html)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Nov 28 2012

Question

The customer want ab13579 with Secondary AB .

Application : WB, IHC both FL & Non FL.

In ab96879 = This is not suitable for WB.

Is it possible to use ab96876 or any one that you can suggest ?

Regards

Read More

Abcam community

Verified customer

Asked on Apr 19 2012

Answer

Thank you for your enquiry.

ab96876 is a Donkey polyclonal anti mouse IgG secondary, and should therefore detect ab13579TRF2 antibody [4A794]which is a mouse IgG1 primary.

As stated on the datasheet, ab96876 has been tested in IHC-P, ICC/IF, Flow Cyt, and WB.

It is therefore tested and guaranteed for western blot and immunohistochemistry on paraffin embedded sections. I am sorry I am not sure what you mean by IHC both FL - Non FL, could you clarify?

I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

Read More

Abcam Scientific Support

Answered on Apr 19 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.