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Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

Submit a review Q&A (2)References (13)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
  • Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
  • Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

Key features and details

  • Donkey Anti-Mouse IgG H&L (DyLight® 550)
  • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP IRDye® 680RD PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (DyLight® 550)
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details

  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    DyLight® 550 replaces DyLight® 549.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550 – this information however will have no impact on your experiments. For more information please refer to DyLight® 550.
ICC/IF
1/50 - 1/500.
Flow Cyt
1/50 - 1/200.
WB
Use at an assay dependent concentration.
Notes
IHC-P
1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550 – this information however will have no impact on your experiments. For more information please refer to DyLight® 550.
ICC/IF
1/50 - 1/500.
Flow Cyt
1/50 - 1/200.
WB
Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

    Formalin-fixed paraffin-embedded sections of human ovarian carcinoma stained for p53 using a mouse anti-p53 (DO-1) antibody, followed by ab96876 at a 1:100 dilution (5 µg/ml). Counterstained with DAPI (blue).

  • Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)Courtesy of an anonymous Abreview
    All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution
    1. Lane 1 : Rat Hippocampus
    2. Lane 2 : Rat Hippocampus
    3. Lane 3 : Rat Hippocampus

    Lysates/proteins at 25 µg per lane.

    Secondary:

    1. Lane 1 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/5000 dilution
    2. Lane 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/1000 dilution
    3. Lane 3 : Other Commercial Dye at 1/1000 dilution

      4. Performed under reducing conditions.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (13)

Publishing research using ab96876? Please let us know so that we can cite the reference in this datasheet.

ab96876 has been referenced in 13 publications.

  • Bos R  et al. Altered action potential waveform and shorter axonal initial segment in hiPSC-derived motor neurons with mutations in VRK1. Neurobiol Dis 164:105609 (2022). PubMed: 34990802
  • Haydn T  et al. Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells. Int J Cancer 146:3435-3449 (2020). PubMed: 31755110
  • Zhang I  et al. Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures. Cells 9:N/A (2020). PubMed: 33171886
  • El-Bazzal L  et al. Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1. Hum Mol Genet 28:2378-2394 (2019). PubMed: 31090908
  • Kinslechner K  et al. Loss of SR-BI Down-Regulates MITF and Suppresses Extracellular Vesicle Release in Human Melanoma. Int J Mol Sci 20:N/A (2019). PubMed: 30823658
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Customer inquired about detecting presence of 7 different proteins in brain tissue via IHC-F. Would like 3-4 different host species for primary, secondary antibodies that do not overlap in spectra. Customer is also not sure if Caspase 3, Nogo, and neurofilament are necessary for detecting apoptosis / neuronal response.

Read More
Answer

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numerous specific published references. The list for these 4 antibodies is given below:
1. GFAP ab4674 (https://www.abcam.com/gfap-antibody-astrocyte-marker-ab4674.html)
2. FOX3 ab104225 (https://www.abcam.com/fox3neun-antibody-ab104225.html)
3. Iba1 ab5076 (https://www.abcam.com/iba1-antibody-ab5076.html)
4. CD68 ab31630 (https://www.abcam.com/cd68-antibody-ed1-ab31630.html)

I also compiled anti-chicken, rabbit, goat, and mouse secondaries with separate emission spectra. We don't have secondaries for these species that weren't raised in any of the 4 species conjugated to a fluorescent protein with a similar spectrum to DAPI. Thus, I think your best bet is to obtain a far-red filter (Cy5), that way you could still stain with DAPI as well. The donkey secondaries that I found with separate emission spectra are listed below:
1. anti-chicken: FITC (https://www.abcam.com/chicken-igy-secondary-antibody-ab63507.html)
2. anti-rabbit: DyLight 594 (Similar spectrum to Texas Red) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-rabbit-igg-h-l-dylight-594-pre-adsorbed-ab96921.html)
3. anti-goat: DyLight 650 (Similar spectrum to Cy5) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-goat-igg-h-l-dylight-650-pre-adsorbed-ab96938.html)
4. anti-mouse: DyLight 550 (similar spectrum to TRITC) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-mouse-igg-h-l-dylight-550-ab96876.html)

The other antibodies you chose (Nogo, heavy neurofilament, caspase 3) seem to be good choices as well as they are tested in formaldehyde fixed, frozen sections via IHC in rat. Below are the antibodies we discussed against those three proteins:
1. anti-Nogo (https://www.abcam.com/nogo-antibody-ab32298.html)
2. anti-heavy neurofilament (https://www.abcam.com/200-kd-neurofilament-heavy-antibody-ab134459.html)
3. anti-caspase 3 (https://www.abcam.com/active-caspase-3-antibody-ab13847.html)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

The customer want ab13579 with Secondary AB .

Application : WB, IHC both FL & Non FL.

In ab96879 = This is not suitable for WB.

Is it possible to use ab96876 or any one that you can suggest ?

Regards

Read More
Answer

Thank you for your enquiry.

ab96876 is a Donkey polyclonal anti mouse IgG secondary, and should therefore detect ab13579TRF2 antibody [4A794]which is a mouse IgG1 primary.

As stated on the datasheet, ab96876 has been tested in IHC-P, ICC/IF, Flow Cyt, and WB.

It is therefore tested and guaranteed for western blot and immunohistochemistry on paraffin embedded sections. I am sorry I am not sure what you mean by IHC both FL - Non FL, could you clarify?

I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

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