Donkey Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98795)

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Neddens J  et al. Phosphorylation of different tau sites during progression of Alzheimer's disease. Acta Neuropathol Commun 6:52 (2018). Read more (PubMed: 29958544) »
  • Javasky E  et al. Study of mitotic chromatin supports a model of bookmarking by histone modifications and reveals nucleosome deposition patterns. Genome Res 28:1455-1466 (2018). Read more (PubMed: 30166406) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

very good

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
excellent
in the picture myofibroblasts stained for alpha-SMA (red) and DAPI (blue)

Abcam user community

Verified customer

Submitted Jun 13 2019

Answer

DISCOUNT CODE: XXXXXX Expiration date: 20-Apr-2012 I am very pleased to hear you would like to accept our offer and test ab9116 in IHC-FR (4% PFA fixed Drosophila sections). This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for IHC-FR and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. ab1274, ab9116 and ab98795 are not covered under Abpromise guarantee as per customer request. I am including other product under IHC-Fr application with PFA as fixative. Ab98795 is a secondary antibody; Abreview discount code is not valid for this product. However if customer provide us Abreview we will assign the Abpoints by manually publishing the Abreview. You can forward the Abreview to me. The Abreview discount code for ab1274 and ab9116 can be provided however customer needs to explain how they have expressed tag conjugated protein in drosophila; please also forward the end user details. Please be also advised that ab1274, ab9116, ab9134, ab84164 are the antibodies against tags. These tags are not naturally present in any cells. These products can only be used in detecting the tag conjugated recombinant proteins that are expressed in tissues or cells. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. These antibodies are against tags so species is not applicable for these products. "10 min in 4% paraformaldehyde and proceeds with the staining" is not enough information that we need for any recommendations. Please ask the customer to provide following info - Are they planning to use tissue sections or cells? What type of tissue section or cells they will be using e.g. Eye, Brain etc. - Are they planning Immunohistochemistry on frozen sections or paraformaldehyde sections thereby incubating sections with acetone or ethanol or treating sections with antigen retrieval method? - Now these antibodies are against tags so you may ask how they are expressing the tag in cells or tissues. Please provide the above information then only we can provide further confirmation.

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