Donkey Anti-Mouse IgG H&L (FITC) (ab97029)
Key features and details
- Donkey Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, Flow Cyt
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Overview
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Product name
Donkey Anti-Mouse IgG H&L (FITC)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, Flow Cytmore details -
Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC. -
Conjugation notes
F/P ratio is 3-7 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/50 - 1/200.
|
|
| IHC-P |
1/50 - 1/500.
|
|
| Flow Cyt |
1/50 - 1/200.
|
| Notes |
|---|
|
ICC
1/50 - 1/200. |
|
IHC-P
1/50 - 1/500. |
|
Flow Cyt
1/50 - 1/200. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (5)
ab97029 has been referenced in 5 publications.
- Nushtaeva AA et al. Establishment of primary human breast cancer cell lines using "pulsed hypoxia" method and development of metastatic tumor model in immunodeficient mice. Cancer Cell Int 19:46 (2019). PubMed: 30858763
- Ugur MR et al. Retained Acetylated Histone Four in Bull Sperm Associated With Fertility. Front Vet Sci 6:223 (2019). PubMed: 31417913
- Tolani B et al. Preclinical characterization of therapeutic antibodies targeted at the carboxy-terminus of Sonic hedgehog. Oncotarget 9:14311-14323 (2018). PubMed: 29581846
- Heck TG et al. Acute exercise boosts cell proliferation and the heat shock response in lymphocytes: correlation with cytokine production and extracellular-to-intracellular HSP70 ratio. Cell Stress Chaperones 22:271-291 (2017). PubMed: 28251488
- Leprieur EG et al. Membrane-bound full-length Sonic Hedgehog identifies cancer stem cells in human non-small cell lung cancer. Oncotarget 8:103744-103757 (2017). PubMed: 29262597
