Application
Western blot
5ug liver lysate (abcam ab29889) 10ug whole cell lysate (human ES cells)
Blocking buffer - 5%BSA/1%OvAlb/PBS (also works with 5% milk powder) for 3hours
Primary antibody - GAPDH (1:10000 expected size 37kDa) 14hours at 4C
Secondary diluted in TBST (1:5000) for 3 hours at room temperature 3hours at room temperature
ECL prime
5 second exposure
Lane 1. Liver lysate
Lane 2. Ladder
Lane 3. Human ES cell lysate
Blocking buffer - 5%BSA/1%OvAlb/PBS (also works with 5% milk powder) for 3hours
Primary antibody - GAPDH (1:10000 expected size 37kDa) 14hours at 4C
Secondary diluted in TBST (1:5000) for 3 hours at room temperature 3hours at room temperature
ECL prime
5 second exposure
Lane 1. Liver lysate
Lane 2. Ladder
Lane 3. Human ES cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Mar 24 2016