Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopymore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native mouse IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/20000.
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
ELISA 1/140000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

References

This product has been referenced in:
  • Kolyada A  et al. Inhibition of thrombotic properties of persistent autoimmune anti-ß2GPI antibodies in the mouse model of antiphospholipid syndrome. Blood 123:1090-7 (2014). Read more (PubMed: 24277078) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. I am sorry to hear that you have been obtaining high background with this antibody. Following contact with the source of this antibody I have determined that the antibody is affinity purified, then cross adsorbed using solid phase adsorption. This means the affinity purified antibody is passed through column(s) bound with listed species of IgGs. The antibodies which cross react with the listed IgGs will bind to the column(s), thus being removed from the final product. The major background in IHC and FACS is from Fc receptors on the cell surface. I think the best way around this problem is to use antibodies specific to the F(ab')2 fragment instead of whole IgG. e.g. ab5766 Mouse IgG antibody H&L - F(ab)2 Fragment Texas Red conjugated, Goat, FACS, IM. I do also think that it is worth inhibiting the peroxidase activity of this enzyme to ensure that the signal that you are observing is not due to the HRP substrate reacting with the endogenous peroxidase activity. I have determined from the source of this antibody that a good starting dilution is 1:1000. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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