Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. Primary dilution.
WB 1/5000 - 1/50000. Colorimetric: Use 1/5,000-1/30,000 dilution; Chemiluminescent: Use 1/10,000-1/50,000 dilution.
ICC Use at an assay dependent dilution.

References

This product has been referenced in:

  • Yannai S  et al. Combinatorial treatment increases IKAP levels in human cells generated from Familial Dysautonomia patients. PLoS One 14:e0211602 (2019). Read more (PubMed: 30889183) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your phone call. I am sending you the following discount codes for trying the antibodies in sELISA and mouse/rat. As for the proteins, I have found the following:

Luteinizing Hormone: none
FSH: ab51888 (human) - test in sELISA
TSH: ab110682 (human) - test in sELISA

Since the FSH and TSH proteins have not yet been tested in sELISA, I am sending you also discount codes for these proteins (see below). Unfortunately, we do not carry the Luteinizing Hormone protein.

In terms of secondary antibodies, you would only need one for the Luteinizing Hormone sELISA as none of these antibodies has an HRP label. The other antibody pairs for TSH and FSH contain an HRP-labeled primary antibody.

The suggested secondary antibodies are:
ab98799 - donkey anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98799.html)
ab98808 - goat anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html)

Testing Discount Codes:

DISCOUNT CODE: xxx- test in mouse, rat
DISCOUNT CODE: xxx- test in mouse, rat
DISCOUNT CODE: xxx- test in sELISA, mouse, rat
DISCOUNT CODE: xxx-test in sELISA, mouse, rat
DISCOUNT CODE: xxx- test in mouse, rat
DISCOUNT CODE: xxx- test in mouse, rat

DISCOUNT CODE: xxx ab51888 (human FSH protein) - test in sELISA
DISCOUNT CODE: xxx ab110682 (human TSH protein) - test in sELISA

Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test thes antibodies and proteins in sELISA and nouse/rat. Each code will give you 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit each an Abreview for sELISA and mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Please also remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Please let me know if there is anything else I can help you with.

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Answer

Thank you for contacting us.

The same antibody but without HRP is ab98791:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98791).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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