Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216778)
Key features and details
- Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed
- Conjugation: IRDye® 680RD
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
Related conjugates and formulations
Alexa Fluor® 405
Alexa Fluor® 555
Alexa Fluor® 568
Alexa Fluor® 647
Alexa Fluor® 680
Alexa Fluor® 750
Alexa Fluor® 790
Alkaline Phosphatase
Biotin
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 650
FITC
Gold 10nm
Gold 12nm
Gold 18nm
Gold 6nm
HRP
PE
Texas Red ®
TRITC
Unconjugated
Unconjugated
Unconjugated
Unconjugated
Overview
-
Product name
Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: WB, In-Cell ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Horse, Human, Rabbit, Sheep, Syrian hamster more details -
Immunogen
Full length native protein (purified) corresponding to Mouse IgG. Whole molecule
-
Conjugation
IRDye® 680RD
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity chromatography purified using antigens coupled to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/25000.
|
|
| In-Cell ELISA |
1/200 - 1/800.
|
| Notes |
|---|
|
WB
1/5000 - 1/25000. |
|
In-Cell ELISA
1/200 - 1/800. |
Images
-
Spectrum - Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216778)
Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab216778 has been referenced in 1 publication.
- Schukken KM et al. Altering microtubule dynamics is synergistically toxic with spindle assembly checkpoint inhibition. Life Sci Alliance 3:N/A (2020). PubMed: 31980556