Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216778)

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Spectrum - Donkey anti-Mouse IgG H&amp;L (IRDye<sup>&reg;</sup> 680RD) preadsorbed (ab216778)

    Key features and details

    • Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed
    • Conjugation: IRDye® 680RD
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: WB, In-Cell ELISA

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 6nm HRP IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

    Overview

    • Product name

      Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed
      See all IgG secondary antibodies

    • Description

      Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed

    • Host species

      Donkey

    • Target species

      Mouse

    • Tested applications

      Suitable for: WB, In-Cell ELISAmore details

    • Minimal
      cross-reactivity


      Chicken, Cow, Goat, Guinea pig, Horse, Human, Rabbit, Sheep, Syrian hamster more details

    • Immunogen

      Full length native protein (purified) corresponding to Mouse IgG. Whole molecule

    • Conjugation

      IRDye® 680RD

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituent: PBS

      Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      Affinity chromatography purified using antigens coupled to agarose.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab216778 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/5000 - 1/25000.
    In-Cell ELISA
    1/200 - 1/800.
    Notes
    WB
    1/5000 - 1/25000.
    In-Cell ELISA
    1/200 - 1/800.

    Images

    • Spectrum - Donkey anti-Mouse IgG H&amp;L (IRDye<sup>&reg;</sup> 680RD) preadsorbed (ab216778)
      Spectrum - Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216778)

      Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab216778? Please let us know so that we can cite the reference in this datasheet.

    ab216778 has been referenced in 1 publication.

    • Schukken KM  et al. Altering microtubule dynamics is synergistically toxic with spindle assembly checkpoint inhibition. Life Sci Alliance 3:N/A (2020). PubMed: 31980556

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