Donkey Anti-Mouse IgG H&L (PE) preadsorbed (ab7003)

Submit a review Q&A (3)References (5)

Key features and details

  • Donkey Anti-Mouse IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ICC/IF

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (PE) preadsorbed
    See all IgG secondary antibodies

  • Description

    Donkey Anti-Mouse IgG H&L (PE) preadsorbed

  • Host species

    Donkey

  • Target species

    Mouse

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ICC/IFmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • Immunogen

    Mouse IgG whole molecule

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

  • Conjugation notes

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/250.
ICC/IF 1/100 - 1/250.

Images

  • Flow Cytometry - Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7003)
    Flow Cytometry - Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed (ab7003)
    Ab7003 was used with the primary antibody ab33315 in Flow Cyt. See Abreview on ab33315.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab7003? Please let us know so that we can cite the reference in this datasheet.

ab7003 has been referenced in 5 publications.

  • Dai T  et al. Analysis of the clinical significance of DCLK1+ colorectal cancer using novel monoclonal antibodies against DCLK1. Onco Targets Ther 11:5047-5057 (2018). PubMed: 30174443
  • Banerjee SR  et al. 111In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues. Biomacromolecules 18:201-209 (2017). PubMed: 28001364
  • Cheng W  et al. Role of the novel HSP90 inhibitor AUY922 in hepatocellular carcinoma: Potential for therapy. Mol Med Rep 12:2451-6 (2015). WB . PubMed: 25955495
  • Zhu J  et al. Effects of the novel heat shock protein 90 inhibitor AUY922 in renal cell carcinoma ACHN and 786-O cells. Oncol Lett 10:941-945 (2015). WB . PubMed: 26622600
  • Marion-Poll L  et al. Fluorescence-activated sorting of fixed nuclei: a general method for studying nuclei from specific cell populations that preserves post-translational modifications. Eur J Neurosci 39:1234-44 (2014). Flow Cyt . PubMed: 24713002

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Hi,
Hope all is well with you. Just wondering if you had any outcome to this investigation yet?
Kind regards,

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Answer

I’m sorry for the delay in getting back to you. I have discussed the problem the customer had been encountering with my colleague and as I mentioned the results they have been observing are what would be expected. The Alexa488 is a much stronger fluorophore than the PE conjugate and would therefore be expected to give a stronger signal. The ab7003 antibody being pre-adsorbed, whilst making it more specific for the particular species of antibody you are hoping to target, will also contribute to it being slightly less signal.
However, if your customer would like, I can offer for her to try an alternative antibody from our catalogue which may prove to give better results. I would suggest one of the following:
Ab99605 this is a rat monoclonal antibody and whilst it is still PE conjugated, it has been specifically enhanced to work in flow applications.
Alternatively, I can suggest altering the fluorochrome used to increase the signal such as using DyLight 650 or Cy5 (ab96882 or ab97037 respectively) if the customer has access to the appropriate lasers for excitation.
I would be willing to send the customer any of the suggested antibodies to try, or an alternative they may prefer. In order to arrange this could you please let me know which antibody they would like, as well as the original order number.
We are closed Monday and Tuesday of next week due the the Queens jubilee celebrations, I am sorry for any inconvenience this may cause.
I look forward to receiving your reply.

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Question

Dear Supplier,

I am trying to lodge a technical inquiry for AB7003. Unfortunately the web page is not allowing me to lodge this inquiry. It keeps requesting a - Please enter the full abcam lot number e.g. GR12345-1. According to our records and customers record lot# is GR1846. I have even tried to put a dud number at the end. Still it won`t let me submit inquiry.

Therefore, please see below the screen shot of the web page. I have attached a technical inquiry form and 2 images. Can you please lodge this inquiry. Let me know if you require any further information.

Kind regards,

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Answer

Thank you for contacting us and sorry for the delay in getting back to you.
I have looked at the protocol your customer has been using and I would say that the results they have been observing are what would be expected. The Alexa488 is a much stronger fluorophore than the PE conjugate and would therefore be expected to give a stronger signal. The ab7003 antibody being pre-adsorbed, whilst making it more specific for the particular species of antibody you are hoping to target, will also contribute to it being slightly less signal.
I am going to speak to one of my collegues who is an expert in this area to find out if we might have a more suitable antibody for the experiment your customer is hoping to perform and will get back to you as soon as I have this information.
In the meantime I wish you a lovely weekend.

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Question

  I would like to ask, in order to be absolutely certain, whether your secondary antibodies, mentioned below, contain Fc part: Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7002) Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (PE), pre-adsorbed (ab7003)   I would like to use them in the experiment where their binding to the Fc receptors would be an important issue.  

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Answer

Thank you for contacting us.  Our F(ab')2 fragment secondary antibodies ab7002 and ab7003 will not contain the Fc region, and this will be covered under our Abpromise guarantee.  If any Fc reactivity is observed in your experiments, we would be happy to offer you a free of charge replacement, credit, or refund.  I hope this helps, please let me know if you need any additional information. 

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