Overview

  • Product name
    Donkey Anti-Mouse IgG H&L (Phycoerythrin) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ICC/IFmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notes
    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/250.
ICC/IF 1/100 - 1/250.

Images

  • Ab7003 was used with the primary antibody ab33315 in Flow Cyt. See Abreview on ab33315.

References

This product has been referenced in:
  • Dai T  et al. Analysis of the clinical significance of DCLK1+ colorectal cancer using novel monoclonal antibodies against DCLK1. Onco Targets Ther 11:5047-5057 (2018). Read more (PubMed: 30174443) »
  • Banerjee SR  et al. 111In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues. Biomacromolecules 18:201-209 (2017). Read more (PubMed: 28001364) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I’m sorry for the delay in getting back to you. I have discussed the problem the customer had been encountering with my colleague and as I mentioned the results they have been observing are what would be expected. The Alexa488 is a much stronger fluorophore than the PE conjugate and would therefore be expected to give a stronger signal. The ab7003 antibody being pre-adsorbed, whilst making it more specific for the particular species of antibody you are hoping to target, will also contribute to it being slightly less signal.
However, if your customer would like, I can offer for her to try an alternative antibody from our catalogue which may prove to give better results. I would suggest one of the following:
Ab99605 this is a rat monoclonal antibody and whilst it is still PE conjugated, it has been specifically enhanced to work in flow applications.
Alternatively, I can suggest altering the fluorochrome used to increase the signal such as using DyLight 650 or Cy5 (ab96882 or ab97037 respectively) if the customer has access to the appropriate lasers for excitation.
I would be willing to send the customer any of the suggested antibodies to try, or an alternative they may prefer. In order to arrange this could you please let me know which antibody they would like, as well as the original order number.
We are closed Monday and Tuesday of next week due the the Queens jubilee celebrations, I am sorry for any inconvenience this may cause.
I look forward to receiving your reply.

Read More

Answer

Thank you for contacting us and sorry for the delay in getting back to you.
I have looked at the protocol your customer has been using and I would say that the results they have been observing are what would be expected. The Alexa488 is a much stronger fluorophore than the PE conjugate and would therefore be expected to give a stronger signal. The ab7003 antibody being pre-adsorbed, whilst making it more specific for the particular species of antibody you are hoping to target, will also contribute to it being slightly less signal.
I am going to speak to one of my collegues who is an expert in this area to find out if we might have a more suitable antibody for the experiment your customer is hoping to perform and will get back to you as soon as I have this information.
In the meantime I wish you a lovely weekend.

Read More

Answer

Thank you for contacting us.  Our F(ab')2 fragment secondary antibodies ab7002 and ab7003 will not contain the Fc region, and this will be covered under our Abpromise guarantee.  If any Fc reactivity is observed in your experiments, we would be happy to offer you a free of charge replacement, credit, or refund.  I hope this helps, please let me know if you need any additional information. 

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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