Key features and details
- Donkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Donkey
- Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IF
Product nameDonkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native mouse IgG (purified).
ConjugationTexas Red ®. Ex: 596nm, Em: 620nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notesTexas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
Our Abpromise guarantee covers the use of ab7059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Immunomicroscopy||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
|ICC/IF||1/1000 - 1/5000. PubMed: 18263878|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7059 has been referenced in 3 publications.
- Muller-Delp JM et al. Effects of age and exercise training on coronary microvascular smooth muscle phenotype and function. J Appl Physiol (1985) 124:140-149 (2018). PubMed: 29025901
- Planutis K et al. A novel signaling pathway regulates colon cancer angiogenesis through Norrin. Sci Rep 4:5630 (2014). IHC-P ; Human . PubMed: 25005225
- Hejna J et al. Tip60 Is Required for DNA Interstrand Cross-link Repair in the Fanconi Anemia Pathway. J Biol Chem 283:9844-51 (2008). ICC/IF . PubMed: 18263878